2019 journal article

Immunohistochemical localization of Cassava brown streak virus and its morphological effect on cassava leaves

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 105, 67–76.

By: M. Saggaf*, J. Ndunguru*, F. Tairo*, P. Sseruwagi*, J. Ascencio-Ibanez  n, D. Kilalo*, D. Miano *

author keywords: Manihot esculenta; Cassava brown streak virus (CBSV); Immunohistochemistry; Cassava brown streak disease; Immunolocalization
TL;DR: Investigation of the localization of Cassava brown streak virus in cassava leaf tissues determined and cellular morphological changes in CBSV-infected tissues were evaluated showed successful localization of CBSV. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
Source: Web Of Science
Added: February 18, 2019

The localization of Cassava brown streak virus (CBSV) in cassava (Manihot esculenta) leaf tissues was determined and cellular morphological changes in CBSV-infected tissues were evaluated. CBSV-symptomatic leaves were screened with CBSV-specific primers using reverse-transcriptase polymerase chain reaction. Immunohistochemical reactions showed precipitation in CBSV-infected but not CBSV-free tissues, demonstrating successful localization of CBSV. Microscopic inspection showed significantly larger (P < 0.001) midribs in CBSV-infected compared with control (uninfected) leaves. Viral accumulation occurred in middle and lower but rarely in young upper leaves. This immunohistochemical method for virus localization will be invaluable for efficient screening of CBSV and for breeding resistant cassava.