2019 journal article
Lack of influence by endosymbiont Wolbachia on virus titer in the common bed bug, Cimex lectularius
PARASITES & VECTORS, 12(1).
author keywords: Cimex lectularius; Wolbachia; Endosymbiont; Cimicidae; Virus suppression; ssRNA
MeSH headings : Animals; Bedbugs / microbiology; Bedbugs / virology; Calicivirus, Feline / growth & development; Calicivirus, Feline / isolation & purification; Microbial Interactions; Viral Load; Wolbachia / growth & development
TL;DR:
It is suggested that other mechanisms are involved in virus suppression within bed bugs, independent of the influence of Wolbachia, and the need for future research is underscore.
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open access via parasitesandvectors.biomedcentral.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: September 30, 2019
AbstractBackgroundThe common bed bug,Cimex lectularius, is an obligatory blood-feeding ectoparasite that requires a blood meal to molt and produce eggs. Their frequent biting to obtain blood meals and intimate association with humans increase the potential for disease transmission. However, despite more than 100 years of inquiry into bed bugs as potential disease vectors, they still have not been conclusively linked to any pathogen or disease. This ecological niche is extraordinarily rare, given that nearly every other blood-feeding arthropod is associated with some type of human or zoonotic disease. Bed bugs rely on the bacteriaWolbachiaas an obligate endosymbiont to biosynthesize B vitamins, since they acquire a nutritionally deficient diet, but it is unknown ifWolbachiaconfers additional benefits to its bed bug host. In some insects,Wolbachiainduces resistance to viruses such as Dengue, Chikungunya, West Nile, Drosophila C and Zika, and primes the insect immune system in other blood-feeding insects.Wolbachiamight have evolved a similar role in its mutualistic association with the bed bug. In this study, we evaluated the influence ofWolbachiaon virus replication withinC. lectularius.MethodsWe used feline calicivirus as a model pathogen. We fed 40 bed bugs from an established line ofWolbachia-cured and a line ofWolbachia-positiveC. lectulariusa virus-laden blood meal, and quantified the amount of virus over five time intervals post-feeding. The antibiotic rifampicin was used to cure bed bugs ofWolbachia.ResultsThere was a significant effect of time post-feeding, as the amount of virus declined by ~90% over 10 days in both groups, but no significant difference in virus titer was observed between theWolbachia-positive andWolbachia-cured groups.ConclusionsThese findings suggest that other mechanisms are involved in virus suppression within bed bugs, independent of the influence ofWolbachia, and our conclusions underscore the need for future research.