1993 journal article
HISTOLOGY OF INVITRO ADVENTITIOUS BUD DEVELOPMENT ON COTYLEDONS AND HYPOCOTYLS OF FRASER FIR
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 118(1), 163–167.
author keywords: ABIES-FRASERI; CHRISTMAS TREES; TISSUE CULTURE; ORGANOGENESIS
TL;DR:
Although adventitious buds did not develop, cotyledons exposed to growth regulators responded differently than cotYledons placed on medium lacking growth regulators, which may explain why the response is less pronounced compared to other conifers when embryonic tissues are used as explants.
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UN Sustainable Development Goal Categories
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
Cotyledons and hypocotyls of Fraser fir [Abies fraseri (Pursh) Poir.] were excised from seeds treated with H2 O2 for 9 days and placed on bud induction medium containing 10 mg BA/liter and 0.01 mg NAA/liter or medium without growth regulators. Although adventitious buds did not develop, cotyledons exposed to growth regulators responded differently than cotyledons placed on medium lacking growth regulators. Cotyledons and hypocotyls responded similarly to growth regulators during the initial phase in culture, but cell divisions ceased in cotyledons, thus preventing meristemoid and subsequent bud development. After 3 days on medium containing growth regulators cell divisions were localized in epidermal and subjacent layers of hypocotyls, whereas similar cell divisions were' not observed in hypocotyls placed on medium without growth regulators. Cell clusters consisting of two to five cells (promeristemoids) were present after 7 days on hypocotyls placed on bud induction medium. In hypocotyls placed on medium without growth regulators, stomata continued to develop and cells within the cortex became vacuolated during the first 2 weeks in culture. All explants were transferred to secondary medium after 3 weeks. Cell clusters continued to enlarge into meristemoids on hypocotyls initially placed on bud induction medium. Gradually, meristemoids developed into buds and cataphylls were observed covering bud meristems. Chemical names used: N -(phenylmethyl)-1 H -purine-6-amine (BA), 1-naphthaleneacetic acid (NAA).