2020 journal article

A Subset of Equine Gastric Squamous Cell Carcinomas Is Associated With Equus Caballus Papillomavirus-2 Infection

VETERINARY PATHOLOGY, 57(3), 427–431.

By: E. Alloway n, K. Linder n, S. May n, T. Rose n, J. DeLay*, S. Bender*, A. Tucker*, J. Luff n

author keywords: horses; squamous cell carcinoma; Equus caballus papillomavirus; oncogenic viruses; stomach; in situ hybridization
MeSH headings : Animals; Carcinoma, Squamous Cell / genetics; Carcinoma, Squamous Cell / pathology; Carcinoma, Squamous Cell / veterinary; Carcinoma, Squamous Cell / virology; DNA, Viral / genetics; Horse Diseases / virology; Horses; In Situ Hybridization / veterinary; Oncogenes / genetics; Papillomaviridae / genetics; Papillomaviridae / isolation & purification; Papillomavirus Infections / pathology; Papillomavirus Infections / veterinary; Polymerase Chain Reaction / veterinary; Stomach / pathology; Stomach Neoplasms / veterinary
TL;DR: Support is provided for a potential association between EcPV-2 infection and a subset of equine gastric SCCs by performing polymerase chain reaction (PCR) and in situ hybridization (ISH) for EcPVs E6/ E7 oncogenes. (via Semantic Scholar)
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Squamous cell carcinoma (SCC) is the most common neoplasm of the equine stomach. However, the mechanisms underlying malignant transformation are unknown. As Equus caballus papillomavirus–2 (EcPV-2) is a likely cause of some genital SCCs, we hypothesized that EcPV-2 is associated with a subset of equine gastric SCCs. To this aim, we performed polymerase chain reaction (PCR) and in situ hybridization (ISH) for EcPV-2 E6/ E7 oncogenes on 11 gastric SCCs and on gastric samples from 15 control horses with no SCC. PCR for EcPV-2 was positive in 7/11 (64%) gastric SCCs; non-SCC gastric samples were all negative. Intense hybridization signals for EcPV-2 E6/E7 nucleic acid were detected by ISH within tumor cells in 5/11 (45%) gastric SCCs, including distant metastases. No hybridization signals were detected within any of the non-SCC gastric cases. This study provides support for a potential association between EcPV-2 infection and a subset of equine gastric SCC.