2021 journal article
Algal Lysis by Sagittula stellata for the Production of Intracellular Valuables
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 193(8), 2516–2533.
author keywords: Microalga; Algicidal bacteria; Cell lysis; Intracellular valuables; Sagittula stellata; Nannochloropsis oceanica
MeSH headings : Microalgae / microbiology; Rhodobacteraceae / growth & development; Stramenopiles / microbiology; Water Microbiology
TL;DR:
The results indicate that Sagittula stellata effectively lysed microalgae cells, allowing the recovery of intracellular valuables, and provides an important framework for the broad application of algicidal microorganisms in algal cell disruption and the production of intrACEllularvaluables.
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UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(Web of Science)
14. Life Below Water
(OpenAlex)
Source: Web Of Science
Added: April 19, 2021
The purpose of this study was to examine the efficacy of the algicidal bacterium Sagittula stellata on the cell lysis of Nannochloropsis oceanica, a microalga found in the marine environment, in order to extract intracellular valuables. Algicidal bacteria are capable of lysing algal cell walls while keeping lipids and proteins intact yet separated. We obtained these microbes from locations with consistent algae blooms and found that the bacterium Sagittula stellata displayed significant algicidal properties toward Nannochloropsis oceanica, achieving an algicidal rate of 80.1%. We detected a decrease of 66.2% in in vivo fluorescence intensity in algae cultures, obtained a recoverable crude lipid content of 23.3% and a polyunsaturated fatty acid (PUFA) ratio of 29.0% of bacteria-treated algae, and observed the lysis of the cell membrane and the structure of the nucleus of algae. We also identified the inhibited transcription of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS) gene and proliferating cell nuclear antigen (PCNA)-related genes and the upregulated heat shock protein (hsp) gene in algal cells during bacterial exposure. Our results indicate that Sagittula stellata effectively lysed microalgae cells, allowing the recovery of intracellular valuables. The algicidal method of Sagittula stellata on Nannochloropsis oceanica cells was confirmed to be a direct attack (or predation), followed by an indirect attack through the secretion of extracellular algicidal compounds. This study provides an important framework for the broad application of algicidal microorganisms in algal cell disruption and the production of intracellular valuables.