2014 journal article

Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus

ARCHIVES OF VIROLOGY, 159(10), 2771–2775.

By: T. Marks n & P. Hamilton n

MeSH headings : Bacillus subtilis / virology; Bacteriophages / genetics; Bacteriophages / isolation & purification; Base Composition; Base Sequence; DNA, Viral / genetics; Genome, Viral / genetics; Geobacillus / virology; Hot Temperature; Molecular Sequence Data; Open Reading Frames / genetics; Sequence Analysis, DNA; Soil Microbiology; Viral Proteins / genetics
TL;DR: The overall genomic arrangement of GBK2 is similar to that of SPP1, with the majority ofGBK2SPP1-like genes coding for proteins involved in DNA replication and metabolism. (via Semantic Scholar)
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GBK2 is a bacteriophage, isolated from a backyard compost pile, that infects the thermophile Geobacillus kaustophilus. GBK2 has a circularly permuted genome of 39,078 bp with a G+C content of 43 %. Annotation of the genome reveals 62 putative open reading frames (ORFs), 25 of which (40.3 %) show homology to known proteins and 37 of which (59.7 %) are proteins with unknown functions. Twelve of the identified ORFs had the greatest homology to genes from the phage SPP1, a phage that infects the mesophile Bacillus subtilis. The overall genomic arrangement of GBK2 is similar to that of SPP1, with the majority of GBK2 SPP1-like genes coding for proteins involved in DNA replication and metabolism.