2022 chapter
Evaluating the Effects of the Circadian Clock and Time of Day on Plant Gravitropic Responses
In Methods in Molecular Biology (pp. 301–319).
author keywords: Circadian; Gravistimulation; Time of day; Diel; Simulated microgravity
MeSH headings : Arabidopsis / metabolism; Arabidopsis Proteins / genetics; Arabidopsis Proteins / metabolism; Circadian Clocks; Circadian Rhythm; Gene Expression Regulation, Plant; Gravitropism; Photoperiod
TL;DR:
It is critical to consider the time of day when performing gravitropism experiments, even if the circadian clock is not a focus of study.
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Added: October 14, 2021
Circadian rhythms are regular oscillations of an organism's physiology with a period of approximately 24 h. In the model plant Arabidopsis thaliana, circadian rhythms regulate a suite of physiological processes, including transcription, photosynthesis, growth, and flowering. The circadian clock and external rhythmic factors have extensive control of the underlying biochemistry and physiology. Therefore, it is critical to consider the time of day when performing gravitropism experiments, even if the circadian clock is not a focus of study. We describe the critical factors and methods to be considered and methods to investigate the possible circadian regulation of gravitropic responses.