2021 journal article

HOPX+ injury-resistant intestinal stem cells drive epithelial recovery after severe intestinal ischemia

American Journal of Physiology-Gastrointestinal and Liver Physiology, 9.

By: A. Stewart n , C. Schaaf  n, J. Luff  n, J. Freund  n, T. Becker *, S. Tufts n, J. Robertson  n, L. Gonzalez  n

author keywords: epithelial repair; HOPX; ischemia; large animal models; stem cell
MeSH headings : Animals; Cell Proliferation; Disease Models, Animal; Epithelial Cells / metabolism; Epithelial Cells / pathology; Female; Homeodomain Proteins / genetics; Homeodomain Proteins / metabolism; Intestinal Mucosa / metabolism; Intestinal Mucosa / pathology; Male; Mesenteric Ischemia / genetics; Mesenteric Ischemia / metabolism; Mesenteric Ischemia / pathology; Phenotype; Re-Epithelialization; Severity of Illness Index; Stem Cells / metabolism; Stem Cells / pathology; Sus scrofa; Tissue Culture Techniques
TL;DR: It is demonstrated that during early in vivo recovery, injury-resistant HOPX+cells maintain quiescence, suggesting that HopX may serve a functional role in ISC mediated regeneration after injury and could be a target to control ISC proliferation. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: ORCID
Added: November 3, 2021

This paper supports that rISCs are resistant to ischemic injury and likely an important source of cellular renewal following near-complete epithelial loss. Furthermore, we have evidence that HOPX controls ISC activity state and may be a critical signaling pathway during ISC-mediated repair. Finally, we use multiple novel methods to evaluate ISCs in a translationally relevant large animal model of severe intestinal injury and provide evidence for the potential role of rISCs as therapeutic targets.