Bacterial etiolation, caused by Acidovorax avenae and Xanthomonas translucens, has become a widespread problem in turfgrass throughout the U.S. Various management tactics are used in managing this disease and differ among turfgrass managers. The use of biostimulants and trinexapac-ethyl (TE) has become a staple in putting green management and many products have been associated with etiolation outbreaks. Experiments performed in field and controlled environments evaluated the impact of commercial biostimulants and TE on etiolation of creeping bentgrass (Agrostis stolonifera L. c.v. ‘Penn A-1’) caused by both A. avenae and X. translucens. In the field, a factorial study was arranged as a split-plot randomized complete block design with 4 replications. The main plot consisted of biostimulants (Knife Plus, CytoGro, Astron, Nitrozyme, PerkUp, BioMax, and none) applied at label rates while the subplot treatments consisted of TE application frequency (0.049 kg ha−1 applied at 7 d, 14 d, and none). For controlled environment experiments, biostimulant and TE treatments were arranged in a randomized complete block design with 4 replications. Bacterial etiolation was measured regularly when present using a grid count to determine the percent area exhibiting etiolation in the field while etiolated turfgrass plants were counted individually in controlled environments. Turf quality was also rated using a scale of 1–9 with 1 = completely dead, 9 = best, and 5 = minimum acceptable turf quality for all experiments. Biostimulant treatments did not have a significant effect on etiolation caused by either bacterium. Trinexapac-ethyl decreased etiolation caused by X. translucens and increased etiolation caused by A. avenae. These results support the necessity of identifying bacteria associated with etiolation as variable effects were observed with TE treatments. These factors should be considered when developing plant growth regulator programs if etiolation is problematic. Future research to evaluate phytohormone production in these bacteria may improve our understanding of etiolation development while improving methods for control.