2012 journal article

Control of vertebrate intraflagellar transport by the planar cell polarity effector Fuz.

The Journal of Cell Biology.

Eric Brooks

MeSH headings : Animals; Cell Polarity / physiology; Cilia / physiology; Flagella / physiology; Humans; Protein Transport; Xenopus; Xenopus Proteins / genetics; Xenopus Proteins / physiology
TL;DR: The planar cell polarity effector Fuz is required for normal particle dynamics of the intraflagellar transport system, specifically in the retrograde transport of proteins. (via Semantic Scholar)
Source: ORCID
Added: March 23, 2022

Cilia play key roles in development and homeostasis, and defects in cilia structure or function lead to an array of human diseases. Ciliogenesis is accomplished by the intraflagellar transport (IFT) system, a set of proteins governing bidirectional transport of cargoes within ciliary axonemes. In this paper, we present a novel platform for in vivo analysis of vertebrate IFT dynamics. Using this platform, we show that the planar cell polarity (PCP) effector Fuz was required for normal IFT dynamics in vertebrate cilia, the first evidence directly linking PCP to the core machinery of ciliogenesis. Further, we show that Fuz played a specific role in trafficking of retrograde, but not anterograde, IFT proteins. These data place Fuz in the small group of known IFT effectors outside the core machinery and, additionally, identify Fuz as a novel cytoplasmic effector that differentiates between the retrograde and anterograde IFT complexes.