2016 journal article

Novel plasmid conferring kanamycin and tetracycline resistance in the turkey-derived Campylobacter jejuni strain 11601MD

PLASMID, 86, 32–37.

By: M. Crespo n, E. Altermann *, J. Olson n, W. Miller *, K. Chandrashekhar  n & S. Kathariou n

author keywords: Campylobacter; C. jejuni; Plasmid; Antibiotic resistance; tet(O); Tetracycline resistance; Kanamycin resistance; aphA-3
MeSH headings : Animals; Anti-Bacterial Agents / pharmacology; Bacterial Proteins / genetics; Base Composition; Base Sequence; Campylobacter jejuni / drug effects; Campylobacter jejuni / genetics; Campylobacter jejuni / isolation & purification; Carrier Proteins / genetics; Conjugation, Genetic / genetics; DNA, Bacterial / genetics; Jejunum / microbiology; Kanamycin / pharmacology; Kanamycin Kinase / genetics; Microbial Sensitivity Tests; North Carolina; Plasmids / genetics; Poultry Diseases / microbiology; Sequence Analysis, DNA; Tetracycline / pharmacology; Tetracycline Resistance / genetics; Turkeys / microbiology
TL;DR: A novel plasmid harboring tet(O) was identified in C. jejuni strain 11601MD, which was isolated from the jejunum of a turkey produced conventionally in North Carolina, and will contribute to a better understanding of the distribution of potentially self-mobilizing plasmids harboring antibiotic resistance determinants in Campylobacter spp. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

In Campylobacter spp., resistance to the antimicrobials kanamycin and tetracycline is frequently associated with plasmid-borne genes. However, relatively few plasmids of Campylobacter jejuni have been fully characterized to date. A novel plasmid (p11601MD; 44,095 nt) harboring tet(O) was identified in C. jejuni strain 11601MD, which was isolated from the jejunum of a turkey produced conventionally in North Carolina. Analysis of the p11601MD sequence revealed the presence of a high-GC content cassette with four genes that included tet(O) and a putative aminoglycoside transferase gene (aphA-3) highly similar to kanamycin resistance determinants. Several genes putatively involved in conjugative transfer were also identified on the plasmid. These findings will contribute to a better understanding of the distribution of potentially self-mobilizing plasmids harboring antibiotic resistance determinants in Campylobacter spp. from turkeys and other sources.