2022 journal article

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The Stickland Reaction Precursor trans-4-Hydroxy-l-Proline Differentially Impacts the Metabolism of Clostridioides difficile and Commensal Clostridia

MSPHERE, 7(2).

By: A. Reed  ⁿ, J. Fletcher ⁿ, Y. Huang ^*, R. Thanissery  ⁿ, A. Rivera ⁿ, R. Parsons  ⁿ, A. Stewart  ⁿ, D. Kountz^* ...and 3 other authors, including 1 NC State author, A. Shen ^*, E. Balskus ^* & C. Theriot ⁿ

author keywords: Clostridioides difficile; hydroxyproline; amino acids; Clostridia; Stickland reaction; colonization resistance; Stickland fermentation

MeSH headings : Animals; Clostridioides; Clostridioides difficile / genetics; Clostridium; Clostridium Infections / metabolism; Hydroxyproline / chemistry; Hydroxyproline / metabolism; Mice; Peptide Hydrolases; Proline / metabolism

TL;DR: A C. difficile ΔhypD mutant mutant was constructed and found that it was modestly impaired in fitness in a mouse model of infection, and was associated with an altered microbiota when compared to mice challenged with the wild-type strain. (via Semantic Scholar)

10.1128/msphere.00926-21

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open access via doi.org (publisher)

PubMed

UN Sustainable Development Goal Categories

3. Good Health and Well-being (Web of Science)

Source: Web Of Science

Added: July 5, 2022

Proline is an essential environmental amino acid that C. difficile uses to support growth and cause significant disease. A posttranslationally modified form, hydroxyproline, is highly abundant in collagen, which is degraded by host proteases in response to C. difficile toxin activity.