2020 journal article

Functional interrelationships between carbohydrate and lipid storage, and mitochondrial activity during sporulation in Saccharomyces cerevisiae

Yeast.

Orlando Arguello-Miranda

author keywords: cyclin 3; glycogen; lipid droplet; meiosis; trehalose; yeast
MeSH headings : Carbohydrates / chemistry; Cyclins / genetics; Cyclins / metabolism; DNA Replication; Gene Expression Regulation, Fungal; Lipids / chemistry; Meiosis; Mitochondria / metabolism; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism; Spores, Fungal / physiology; Transcriptome
TL;DR: Results provide new insights into the complex crosstalk between metabolic factors that support gametogenesis and storage carbohydrate‐deficient strains exhibited a greater dependency on mitochondrial activity and lipid stores than wild‐type yeast. (via Semantic Scholar)
Source: ORCID
Added: July 11, 2022

AbstractIn Saccharomyces cerevisiae under conditions of nutrient stress, meiosis precedes the formation of spores. Although the molecular mechanisms that regulate meiosis, such as meiotic recombination and nuclear divisions, have been extensively studied, the metabolic factors that determine the efficiency of sporulation are less understood. Here, we have directly assessed the relationship between metabolic stores and sporulation in S. cerevisiae by genetically disrupting the synthetic pathways for the carbohydrate stores, glycogen (gsy1/2Δ cells), trehalose (tps1Δ cells), or both (gsy1/2Δ and tps1Δ cells). We show that storage carbohydrate‐deficient strains are highly inefficient in sporulation. Although glycogen and trehalose stores can partially compensate for each other, they have differential effects on sporulation rate and spore number. Interestingly, deletion of the G1 cyclin, CLN3, which resulted in an increase in cell size, mitochondria and lipid stores, partially rescued meiosis progression and spore ascus formation but not spore number in storage carbohydrate‐deficient strains. Sporulation efficiency in the carbohydrate‐deficient strain exhibited a greater dependency on mitochondrial activity and lipid stores than wild‐type yeast. Taken together, our results provide new insights into the complex crosstalk between metabolic factors that support gametogenesis.