2017 journal article
Vitreous Humor Changes Expression of Iron-Handling Proteins in Lens Epithelial Cells
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 58(2).
author keywords: iron; oxidative damage; ferritin; transferrin receptor; vitreous humor
MeSH headings : Animals; Aquaporins / metabolism; Blotting, Western; Cells, Cultured; Disease Models, Animal; Dogs; Epithelial Cells / metabolism; Eye Proteins / metabolism; Ferritins / metabolism; Immunohistochemistry; Lens, Crystalline / metabolism; Receptors, Transferrin / metabolism; Vitreous Body / physiology
TL;DR:
Virreous humor may significantly limit iron uptake by transferrin/transferrin receptor pathway, and by increasing ferritin levels could profoundly increase the iron-storage capacity of ferrit in lens cells.
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open access via iovs.arvojournals.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
Purpose In humans, vitrectomy is associated with development of nuclear cataracts. Iron catalyzes free radical formation causing oxidative damage, which is implicated in cataract formation. This study was designed to determine if vitreous humor, which can initiate differentiation of lens epithelial cells, would have an effect on iron-handling proteins. Methods Cultured canine lens epithelial cells were treated with collected canine vitreous humor. Lysates of treated and control cells were separated by SDS-PAGE. Ferritin H- and L-chains, transferrin receptor 1, and aquaporin 0 were immunodetected and quantitated with specific antibodies. Morphologic changes in treated cells were assessed. Results Treatment of lens epithelial cells with a 33% (vol/vol) solution of vitreous humor changed the morphology of lens cells and induced expression of aquaporin 0, a marker of fiber cell differentiation that was undetectable in control cells. Treatment did not modify the size of iron-handling proteins but significantly increased content of ferritin from 2.9- to 8.8-fold over control and decreased levels of transferrin receptor by 37% to 59%. Conclusions Vitreous humor may significantly limit iron uptake by transferrin/transferrin receptor pathway, and by increasing ferritin levels could profoundly increase the iron-storage capacity of ferritin in lens cells. Vitreous humor may play a significant protective role against iron-catalyzed oxidative damage of lens epithelial cells and therefore in the formation of cataracts.