2022 journal article

Culture of endometrial epithelial cells collected by a cytological brush in vivo

JDS Communications, 3(3), 217–221.

By: C. Rocha *, F. Silva *, T. Martins *, M. Marrero *, J. Bromfield *, J. Driver *, T. Hansen *, L. Oliveira *, M. Binelli *

TL;DR: This work validated an approach to culture and propagate luminal endometrial cells harvest by a minimally invasive tool,Endometrial cytology (cytobrush), by inducing transcription ofIFN-stimulated genes in response to treatment with recombinant IFN-τ. (via Semantic Scholar)
Source: ORCID
Added: January 26, 2023

Graphical Abstract Summary: Understanding endometrial physiology in dairy cows is essential, as approximately 50% of pregnancies are lost in the first 3 weeks of gestation for unknown reasons. This work validated an approach to culture and propagate luminal endometrial cells harvest by a minimally invasive tool, endometrial cytology (cytobrush). (1) Cells were collected at a specific stage of the estrous cycle; (2) fresh cells were evaluated regarding their identity by immunofluorescence; and (3) functionality of the system was demonstrated by inducing transcription of IFN-stimulated genes (ISGs) in response to treatment with recombinant IFN-τ.