2023 article

Lipidomic Analysis of Mouse Brain to Evaluate the Efficacy and Preservation of Different Tissue Preparatory Techniques by IR-MALDESI-MSI

Wang, M. F., Sohn, A. L., Samal, J., Erning, K., Segura, T., & Muddiman, D. C. (2023, March 29). JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY.

By: M. Wang n, A. Sohn n, J. Samal*, K. Erning*, T. Segura * & D. Muddiman n 

co-author countries: United States of America πŸ‡ΊπŸ‡Έ
author keywords: IR-MALDESI; mass spectrometry imaging; sucrose; PFA
MeSH headings : Mice; Animals; Spectrometry, Mass, Electrospray Ionization / methods; Lipidomics; Ice; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods; Ions / chemistry; Lipids / analysis; Brain
Source: Web Of Science
Added: April 24, 2023

Numerous preparatory methods have been developed to preserve the cellular and structural integrity of various biological tissues for different -omics studies. Herein, two preparatory methods for mass spectrometry imaging (MSI) were evaluated, fresh-frozen and sucrose-embedded, paraformaldehyde (PFA) fixed, in terms of ion abundance, putative lipid identifications, and preservation of analyte spatial distributions. Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)-MSI was utilized to compare the preparatory methods of interest with and without the use of the conventional ice matrix. There were 2.5-fold and 1.6-fold more lipid species putatively identified in positive- and negative-ion modes, respectively, for sucrose-embedded, PFA-fixed tissues without an ice matrix relative to the current IR-MALDESI-MSI gold-standard, fresh-frozen tissue preparation with an exogenous ice matrix. Furthermore, sucrose-embedded tissues demonstrated improved spatial distribution of ions resulting from the cryo-protective property of sucrose and paraformaldehyde fixation. Evidence from these investigations supports sucrose-embedding without ice matrix as an alternative preparatory technique for IR-MALDESI-MSI.