2018 journal article

Carbon-Fiber Microbiosensor for Monitoring Rapid Lactate Fluctuations in Brain Tissue Using Fast-Scan Cyclic Voltammetry

ANALYTICAL CHEMISTRY, 90(21), 12994–12999.

By: S. Smith n, S. Gosrani n, C. Lee n, G. McCarty n & L. Sombers n

co-author countries: United States of America 🇺🇸
MeSH headings : Animals; Biosensing Techniques / methods; Carbon Fiber / chemistry; Electrochemical Techniques / instrumentation; Electrochemical Techniques / methods; Enzymes, Immobilized / chemistry; Lactic Acid / analysis; Limit of Detection; Male; Microelectrodes; Mixed Function Oxygenases / chemistry; Neostriatum / metabolism; Rats, Sprague-Dawley; Sensitivity and Specificity
Source: Web Of Science
Added: November 26, 2018

Recent studies have described a role for lactate in brain energy metabolism and energy formation, challenging the conventional view that glucose is the principle energy source for brain function. To date, lactate dynamics in the brain are largely unknown, limiting insight into function. We addressed this by developing and characterizing a lactate oxidase-modified carbon-fiber microelectrode coupled with fast-scan cyclic voltammetry. This new tool boasts a sensitivity for lactate of 22 ± 1 nA·mM–1 and LOD of 7.0 ± 0.7 μM. The approach has enabled detection of rapid lactate fluctuations with unprecedented spatiotemporal resolution as well as excellent stability, selectivity, and sensitivity. The technology was characterized both in vitro and in vivo at discrete recording sites in rat striatum. We provide evidence that striatal lactate availability increases biphasically in response to electrical stimulation of the dopaminergic midbrain in the anesthetized rat. This new tool for real-time detection of lactate dynamics promises to improve understanding of how lactate availability underscores neuronal function and dysfunction.