2023 journal article
A myristoylated alanine-rich C-kinase substrate (MARCKS) inhibitor peptide attenuates neutrophil outside-in β 2 -integrin activation and signaling
Cell Adhesion & Migration.
MeSH headings : Alanine; CD18 Antigens / metabolism; Neutrophils; Peptides / pharmacology; Signal Transduction; Myristoylated Alanine-Rich C Kinase Substrate / antagonists & inhibitors
TL;DR:
MANS treatment significantly attenuated ICAM-1/Mn2+-stimulated static adhesion, cell spreading andβ2-integrin clustering, suggesting a role for MARCKS function in outside-in β2-Integrin activation.
(via Semantic Scholar)
open access via www.tandfonline.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: July 13, 2023
ABSTRACT MARCKS is an actin and PIP2-binding protein that plays an essential role in neutrophil migration and adhesion; however, the molecular details regarding MARCKS function in these processes remains unclear. Neutrophil adhesion and migration also require the cell surface receptors β2-integrins. We hypothesized that MARCKS inhibition would alter neutrophil β2-integrin activation and signaling. We utilized a MARCKS-targeting peptide to inhibit MARCKS in inside-out and outside-in β2-integrin activation in neutrophils. MANS-mediated MARCKS inhibition had no significant effect on inside-out β2-integrin activation. MANS treatment significantly attenuated ICAM-1/Mn2+-stimulated static adhesion, cell spreading and β2-integrin clustering, suggesting a role for MARCKS function in outside-in β2-integrin activation. Additional work is needed to better understand the molecular mechanisms of MARCKS role in outside-in β2-integrin activation and signaling.