2023 article

Tissues and mechanisms associated with Verticillium wilt resistance in tomato using bi-grafted near-isogenic lines

Oh, Y., Ingram, T., Shekasteband, R., Adhikari, T., Louws, F. J., & Dean, R. A. (2023, May 15). JOURNAL OF EXPERIMENTAL BOTANY.

By: Y. Oh n, T. Ingram n, R. Shekasteband n , T. Adhikari n , F. Louws n  & R. Dean n 

author keywords: Defense response; gene expression; grafting; secreted proteins; tomato; Verticillium dahliae
TL;DR: These findings advance knowledge of molecular mechanisms underlying the tomato–V. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: July 19, 2023

Abstract Host resistance is the primary means to control Verticillium dahliae, a soil-borne pathogen causing major losses on a broad range of plants, including tomato. The tissues and mechanisms responsible for resistance remain obscure. In the field, resistant tomato used as rootstocks does not confer resistance. Here, we created bi-grafted plants with near-isogenic lines (NILs) exhibiting (Ve1) or lacking (ve1) resistance to V. dahliae race 1. Ten days after inoculation, scion and rootstock tissues were subjected to differential gene expression and co-expression network analyses. Symptoms only developed in susceptible scions regardless of the rootstock. Infection caused more dramatic alteration of tomato gene expression in susceptible compared with resistant tissues, including pathogen receptor, signaling pathway, pathogenesis-related protein, and cell wall modification genes. Differences were observed between scions and rootstocks, primarily related to physiological processes in these tissues. Gene expression in scions was influenced by the rootstock genotype. A few genes were associated with the Ve1 genotype, which was independent of infection or tissue type. Several were physically clustered, some near the Ve1 locus on chromosome 9. Transcripts mapped to V. dahliae were dominated by secreted candidate effector proteins. These findings advance knowledge of molecular mechanisms underlying the tomato–V. dahliae interaction.