2022 journal article

Activating transcription factor 6 protects against endothelial barrier dysfunction.

Cellular Signalling, 11.

co-author countries: United States of America 🇺🇸
MeSH headings : Actin Depolymerizing Factors / metabolism; Activating Transcription Factor 6 / metabolism; Activating Transcription Factor 6 / pharmacology; Animals; Cattle; Cells, Cultured; Dextrans / metabolism; Dextrans / pharmacology; Endothelial Cells / metabolism; Fluorescein-5-isothiocyanate / analogs & derivatives; Fluorescein-5-isothiocyanate / metabolism; Fluorescein-5-isothiocyanate / pharmacology; Lipopolysaccharides / metabolism; Lipopolysaccharides / pharmacology; RNA, Small Interfering / metabolism; Respiratory Distress Syndrome; Sepsis
Source: ORCID
Added: August 2, 2023

Endothelial hyperpermeability is associated with sepsis and acute respiratory distress syndrome (ARDS). The identification of molecular pathways involved in barrier dysfunction; may reveal promising therapeutic targets to combat ARDS. Unfolded protein response (UPR) is a highly conserved molecular pathway, which ameliorates endoplasmic reticulum stress. The present work focuses on the effects of ATF6, which is a UPR sensor, in lipopolysaccharides (LPS)-induced endothelial hyperpermeability. The in vitro effects of AA147 and Ceapin-A7 in LPS-induced endothelial barrier dysfunction were investigated in bovine pulmonary artery endothelial cells (BPAEC). Small interfering (si) RNA was utilized to “silence” ATF6, and electric cell-substrate impedance sensing (ECIS) measured transendothelial resistance. Fluorescein isothiocyanate (FITC)-dextran assay was utilized to assess paracellular permeability. Protein expression levels were evaluated with Western blotting, and cell viability with MTT assay. We demonstrated that AA147 prevents LPS-induced barrier disruption by counteracting Cofilin and myosin light chain 2 (MLC2) activation, as well as VE-Cadherin phosphorylation. Moreover, this ATF6 inducer opposed LPS-triggered decrease in transendothelial resistance (TEER), as well as LPS-induced paracellular hyperpermeability. On the other hand, ATF6 suppression due to Ceapin-A7 or small interfering RNA exerted the opposite effects, and potentiated LPS-induced endothelial barrier disruption. Moderate concentrations of both ATF6 modulators did not affect cell viability. ATF6 activation protects against endothelial barrier function, suggesting that this UPR sensor may serve as a therapeutic target for sepsis and ARDS.