Bacterial spot (Xanthomonas spp.) is one of the most detrimental diseases of tomato (Solanum lycopersicum L.) worldwide. Bacterial spot Xanthomonads are either endemic or spread by contaminated seed or transplant material into tomato production areas. Due to limited disease management options, transplant producers rely on copper- and streptomycin-based bactericides that are often ineffective due to the predominance of resistant Xanthomonas strains. In this study, transplant production facilities located across eight counties in western North Carolina were surveyed for bacterial spot symptoms from 2018 to 2019. In total, 29 strains (n = 6 in 2018 and n = 23 in 2019) were isolated and identified as Xanthomonas perforans. All strains were resistant to copper, while only 24% of the strains were resistant to streptomycin. Therefore, the ability of three systemic acquired resistance (SAR) inducers, methyl salicylate, Bacillus mycoides isolate J, and acibenzolar-S-methyl (ASM), alone or in combination with copper or streptomycin were evaluated as potential alternatives to copper-mancozeb (grower standard) to manage bacterial spot under greenhouse and field conditions. Under greenhouse conditions, applications of ASM at 0.4 g/L alone, or in combination with copper or streptomycin, significantly reduced bacterial spot disease severity when compared to the grower standard and nontreated controls (P < 0.05). However, none of the SAR inducers significantly reduced disease severity or improved yield when compared to copper-mancozeb-ASM under field conditions (P < 0.05). This study highlights the potential of using ASM for the management of bacterial spot of tomato transplant production to reduce bacterial spot introduction into the field.