Starting in 1573 with the establishment of Manila, the circulation of Chinese goods along the trans-Pacific route brought the Ming Dynasty and the Spanish Empire into contact. Consequently, many people from Fujian Province, especially Zhangzhou city, went to Manila and facilitated the operation of the city. This paper examines the interactions of Zhangzhou immigrants in Manila, focusing their roles and living situations in Parián, the designated neighborhood for the Sangleys. In particular, the diplomatic conversation concerning the 1603 Incident is analyzed to elucidate how the Sangleys were perceived by both the Ming court and the Spanish governors. Records produced by Chinese elites, local gazetteers, and Spanish correspondence reveal the conflicts and negotiations between the Ming government and officials of the Spanish Empire concerning this matter, as well as local and state differences in views. While the contributions of the Sangleys were recognized and used to promote the moral superiority of both sides, they were not seriously considered by either the Chinese court or the Spanish government. The Sangleys offer a unique perspective on the positioning of these two state powers: Each desired to maintain a peaceful relationship yet simultaneously adopted a cautious stance. This study illuminates some of the connections and conflicts which emerged during early modern globalization.