2018 journal article
A Comparison of the chemical sensitivities between in vitro and in vivo propagated juvenile freshwater mussels: Implications for standard toxicity testing
ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 37(12), 3077–3085.
author keywords: Mussel propagation; Copper; Nickel; Ammonia; Unionid
MeSH headings : Aging / physiology; Animals; Bivalvia / drug effects; Fresh Water; Larva / drug effects; Reference Standards; Toxicity Tests, Acute; Water Pollutants, Chemical / toxicity; Water Quality
TL;DR:
In vitro propagated juvenile mussels may be appropriate for use in ASTM International‐based toxicity testing, and differences in chemical sensitivity exist between in vitro and in vivo propagated juveniles, they are within normal toxicity test variation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
14. Life Below Water
(Web of Science)
15. Life on Land
(Web of Science)
Source: Web Of Science
Added: December 17, 2018
AbstractUnionid mussels are ecologically important and are globally imperiled. Toxicants contribute to mussel declines, and toxicity tests using juvenile mussels—a sensitive life stage—are valuable in determining thresholds used to set water quality criteria. In vitro culture methods provide an efficient way to propagate juveniles for toxicity testing, but their relative chemical sensitivity compared with in vivo propagated juveniles is unknown. Current testing guidelines caution against using in vitro cultured juveniles until this sensitivity is described. Our objective was to evaluate the relative sensitivity of juvenile mussels produced from both in vitro and in vivo propagation methods to selected chemicals. We conducted 96‐h acute toxicity tests according to ASTM International guidelines with 3 mussel species and 6 toxicants: chloride, nickel, ammonia, and 3 copper‐based compounds. Statistically significant differences between in vitro and in vivo juvenile 96‐h median effect concentrations were observed in 8 of 17 tests, and in vitro juveniles were more sensitive in 6 of the 8 significant differences. At 96 h, 4 of the 8 statistically different tests for a given chemical were within a factor of 2, which is the intralaboratory variation demonstrated in a recent evaluation of mussel toxicity tests. We found that although differences in chemical sensitivity exist between in vitro and in vivo propagated juvenile mussels, they are within normal toxicity test variation. Therefore, in vitro propagated juvenile mussels may be appropriate for use in ASTM International‐based toxicity testing. Environ Toxicol Chem 2018;37:3077–3085. © 2018 SETAC