2018 journal article

The prevalent Boxer MHC class Ia allotype dog leukocyte antigen (DLA)-88*034:01 preferentially binds nonamer peptides with a defined motif

HLA, 92(6), 403–407.

By: P. Nemec n, A. Kapatos n, J. Holmes n & P. Hess n

co-author countries: United States of America 🇺🇸
author keywords: amino acids; binding motif; dogs; MHC class I; peptides; tandem mass spectrometry
MeSH headings : Amino Acid Motifs; Amino Acids / chemistry; Amino Acids / immunology; Animals; Antigens, Neoplasm / analysis; Antigens, Neoplasm / genetics; Antigens, Neoplasm / immunology; Dogs; Epitopes / chemistry; Epitopes / genetics; Epitopes / immunology; Gene Expression; Histocompatibility Antigens Class I / chemistry; Histocompatibility Antigens Class I / genetics; Histocompatibility Antigens Class I / immunology; Oligopeptides / chemistry; Oligopeptides / genetics; Oligopeptides / immunology; Protein Binding; T-Lymphocytes / chemistry; T-Lymphocytes / immunology; Tandem Mass Spectrometry
Source: Web Of Science
Added: January 14, 2019

Development of effective immunotherapy for chemoresistant malignancies can be advanced by studies in spontaneous cancer models, such as the dog. A crucial first step, T‐cell epitope discovery, can be assisted by determination of binding motifs of common dog leukocyte antigen (DLA) class Ia allotypes. Boxers are popular, inbred dogs with increased risks of relevant target cancers and restricted MHC diversity. We sought to identify the motif of DLA‐88*034:01 , a breed‐dominant allotype, to assist peptide prediction from tumor antigens. Mass spectrometry of eluted peptides showed a preference for nonamers with conserved amino acid preferences: basic at position (P)1; hydrophobic at P2; acidic at P4; histidine at P6; and phenylalanine at P9. This data should expedite finding epitopes restricted by this DLA‐88 allotype.