2008 journal article
Differential activation of dual promoters alters D beta 2 germline transcription during thymocyte development
JOURNAL OF IMMUNOLOGY, 180(5), 3218–3228.
MeSH headings : Animals; Base Sequence; Cell Differentiation / genetics; Cell Differentiation / immunology; Cell Line; Cell Line, Tumor; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics; Germ-Line Mutation / immunology; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Data; Promoter Regions, Genetic / immunology; Receptors, Antigen, T-Cell, alpha-beta / genetics; Receptors, Antigen, T-Cell, alpha-beta / metabolism; Thymus Gland / cytology; Thymus Gland / enzymology; Thymus Gland / immunology; Thymus Gland / metabolism; Transcription, Genetic / immunology; Transcriptional Activation / immunology; VDJ Recombinases / genetics
TL;DR:
The unique organization and timing of Dβ2 promoter activity are consistent with a model in which promoter placement selectively regulates the rearrangement potential of D β2 during TCR β locus assembly.
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open access via www.jimmunol.org (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
Abstract Ag receptor genes are assembled through somatic rearrangements of V, D, and J gene segments. This process is directed in part by transcriptional enhancers and promoters positioned within each gene locus. Whereas enhancers coordinate reorganization of large chromatin stretches, promoters are predicted to facilitate the accessibility of proximal downstream gene segments. In TCR β locus, rearrangement initiates at two D-J cassettes, each of which exhibits transcriptional activity coincident with DJ rearrangement in CD4/CD8 double-negative pro-T cells. Consistent with a model of promoter-facilitated recombination, assembly of the DJβ1 cassette is dependent on a Dβ1 promoter (PDβ1) positioned immediately 5′ of the D. Assembly of DJβ2 proceeds independent from that of DJβ1, albeit with less efficiency. To gain insight into the mechanisms that selectively alter D usage, we have defined transcriptional regulation at Dβ2. We find that both DJβ cassettes generate germline messages in murine CD44+CD25− double-negative 1 cells. However, transcription of unrearranged DJβ2 initiates at multiple sites 400–550 bp downstream of the Dβ2. Unexpectedly, loci from which germline promoter activity has been deleted by DJ rearrangement redirect transcription to sites immediately 5′ of the new DJβ2 joint. Our analyses suggest that 3′-PDβ2 activity is largely controlled by NF-κB RelA, whereas 5′-PDβ2 activity directs germline transcription of DJβ2 joints from initiator elements 76 bp upstream of the Dβ2 5′ recombination signal sequence. The unique organization and timing of Dβ2 promoter activity are consistent with a model in which promoter placement selectively regulates the rearrangement potential of Dβ2 during TCR β locus assembly.