2020 journal article

Monitoring Ethylene in Plants: Genetically Encoded Reporters and Biosensors

Small Methods.

By: J. Fernandez‐Moreno n & A. Stepanova n

co-author countries: United States of America 🇺🇸
author keywords: biosensors; ethylene; hormones; reporters; signaling
Source: ORCID
Added: December 19, 2019

Abstract Phytohormone ethylene regulates numerous aspects of plant physiology, from fruit ripening to pathogen responses. The molecular basis of ethylene biosynthesis and action has been investigated for over 40 years, and a combination of biochemistry, genetics, cell, and molecular biology have proven successful at uncovering the core machinery of the ethylene pathway. A number of molecular tools have been developed over the years that enable visualization of the sites of ethylene production and response in the plant. Genetically encoded biosensors take advantage of reporter proteins, i.e., fluorescent, luminescent, or colorimetric markers, to highlight the tissues that make, perceive, or respond to the hormone. This review describes the different types of biosensors currently available to the ethylene community and discusses potential new strategies for developing the next generation of genetically encoded ethylene reporters.