2019 journal article
Recognition of Histone Crotonylation by Taf14 Links Metabolic State to Gene Expression
MOLECULAR CELL, 76(6), 909-+.
MeSH headings : Acyl Coenzyme A / metabolism; Energy Metabolism / genetics; Fatty Acids / metabolism; Gene Expression Regulation, Fungal; Histones / genetics; Histones / metabolism; Homeostasis; Lysine; Oxidation-Reduction; Protein Processing, Post-Translational; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / genetics; Saccharomyces cerevisiae Proteins / metabolism; Signal Transduction; Transcription Factor TFIID / genetics; Transcription Factor TFIID / metabolism; Transcription, Genetic
TL;DR:
These findings expose an unexpected link between metabolic flux and transcription and demonstrate that histone crotonylation and Taf14 participate in the repression of energy-demanding gene expression.
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UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Source: NC State University Libraries
Added: January 7, 2020
Metabolic signaling to chromatin often underlies how adaptive transcriptional responses are controlled. While intermediary metabolites serve as co-factors for histone-modifying enzymes during metabolic flux, how these modifications contribute to transcriptional responses is poorly understood. Here, we utilize the highly synchronized yeast metabolic cycle (YMC) and find that fatty acid β-oxidation genes are periodically expressed coincident with the β-oxidation byproduct histone crotonylation. Specifically, we found that H3K9 crotonylation peaks when H3K9 acetylation declines and energy resources become limited. During this metabolic state, pro-growth gene expression is dampened; however, mutation of the Taf14 YEATS domain, a H3K9 crotonylation reader, results in de-repression of these genes. Conversely, exogenous addition of crotonic acid results in increased histone crotonylation, constitutive repression of pro-growth genes, and disrupted YMC oscillations. Together, our findings expose an unexpected link between metabolic flux and transcription and demonstrate that histone crotonylation and Taf14 participate in the repression of energy-demanding gene expression.