An Improved Recombineering Toolset for Plants
The Plant Cell.
MeSH headings : Arabidopsis / genetics; Chromosomes, Artificial, Bacterial; Gene Editing / methods; Genes, Reporter; Genetic Engineering / methods; Plants / genetics; Plants, Genetically Modified / genetics; Recombination, Genetic
TL;DR:
A scalable recombinase-mediated cassette exchange system to facilitate the transfer of the desired sequences from a bacterial artificial chromosome clone to a transformation-compatible binary vector is developed, expanding the use of the recombineering approaches beyond Arabidopsis (Arabidopsis thaliana).
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