2016 journal article

Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast

Proceedings of the National Academy of Sciences, 113(40), E5876–E5885.

co-author countries: United States of America πŸ‡ΊπŸ‡Έ
author keywords: super resolution; cytokinesis; cytokinetic nodes; contractile ring; fission yeast
MeSH headings : Actin Cytoskeleton / metabolism; Actins / metabolism; Cytokinesis; Interphase; Microscopy, Fluorescence / methods; Models, Molecular; Phenotype; Schizosaccharomyces / cytology; Schizosaccharomyces / metabolism; Schizosaccharomyces pombe Proteins / metabolism
Source: Crossref
Added: February 24, 2020

Significance Cell division occurs by the assembly and constriction of a ring of actin and myosin; however, the organization of proteins within cytokinetic apparatus is still unknown. Without better information about the organization of contractile rings, we cannot understand how they assemble or constrict. In fission yeast, cytokinetic proteins are first recruited around the equator as cortical spots, called nodes, that coalesce into a ring. Here we used high-speed quantitative fluorescence photoactivation localization microscopy to obtain a molecular model of this basic cytokinetic unit. Nodes are discrete structures with distinct distributions of six different proteins. Nodes persist in contractile rings and move around the circumference as the ring constricts.