2012 journal article

Future Challenges in Single-Molecule Fluorescence and Laser Trap Approaches to Studies of Molecular Motors

Developmental Cell, 23(6), 1084–1091.

co-author countries: United States of America πŸ‡ΊπŸ‡Έ
MeSH headings : Cell Movement; Fluorescence; Microscopy, Atomic Force; Microscopy, Fluorescence; Molecular Motor Proteins / chemistry; Molecular Motor Proteins / metabolism; Optical Tweezers
Source: Crossref
Added: February 24, 2020

Single-molecule analysis is a powerful modern form of biochemistry, in which individual kinetic steps of a catalytic cycle of an enzyme can be explored in exquisite detail. Both single-molecule fluorescence and single-molecule force techniques have been widely used to characterize a number of protein systems. We focus here on molecular motors as a paradigm. We describe two areas where we expect to see exciting developments in the near future: first, characterizing the coupling of force production to chemical and mechanical changes in motors, and second, understanding how multiple motors work together in the environment of the cell.