2018 journal article
Determination of the Acid Dissociation Constant of a Phenolic Acid by High Performance Liquid Chromatography: An Experiment for the Upper Level Analytical Chemistry laboratory
Journal of Chemical Education, 95(2), 310–314.
author keywords: Upper-Division Undergraduate; Analytical Chemistry; Inquiry-Based/Discovery Learning; Hands-On Learning/Manipulatives; Chromatography; HPLC
topics (OpenAlex): Various Chemistry Research Topics; Analytical Chemistry and Chromatography; Chemical and Physical Properties in Aqueous Solutions
UN Sustainable Development Goal Categories
6. Clean Water and Sanitation
(OpenAlex)
Source: ORCID
Added: January 26, 2021
A high performance liquid chromatography (HPLC) experiment for the upper level analytical chemistry laboratory is described. The students consider the effect of mobile-phase composition and pH on the retention times of ionizable compounds in order to determine the acid dissociation constant, Ka, of a phenolic acid. Results are analyzed using nonlinear regression.