2021 journal article

Complement-Opsonized HIV Modulates Pathways Involved in Infection of Cervical Mucosal Tissues: A Transcriptomic and Proteomic Study.

Frontiers in Immunology.

By: C. Svanberg*, R. Ellegård*, E. Crisci*, M. Khalid*, W. Borendal*, M. Svenvik*, S. Nyström*, K. Birse* ...

co-author countries: Canada 🇨🇦 India 🇮🇳 Sweden 🇸🇪 United States of America 🇺🇸
author keywords: HIV; human immunodeficiency virus; innate immunity; complement opsonized HIV-1; transcriptomics; proteomics; cervical tissue; primary infection
MeSH headings : Cervix Uteri / immunology; Cervix Uteri / metabolism; Cervix Uteri / virology; Complement System Proteins / immunology; Dendritic Cells / immunology; Dendritic Cells / metabolism; Dendritic Cells / virology; Female; Gene Expression Profiling; Gene Expression Regulation; HIV Infections / genetics; HIV Infections / immunology; HIV Infections / metabolism; HIV Infections / virology; HIV-1 / growth & development; HIV-1 / immunology; HIV-1 / pathogenicity; Host-Pathogen Interactions; Humans; Immunity, Innate; Mucous Membrane / immunology; Mucous Membrane / metabolism; Mucous Membrane / virology; Proteome; Proteomics; Signal Transduction; T-Lymphocytes / immunology; T-Lymphocytes / metabolism; T-Lymphocytes / virology; Time Factors; Tissue Culture Techniques; Transcriptome; Virus Internalization; Virus Replication
Source: ORCID
Added: July 1, 2021

Genital mucosal transmission is the most common route of HIV spread. The initial responses triggered at the site of viral entry are reportedly affected by host factors, especially complement components present at the site, and this will have profound consequences on the outcome and pathogenesis of HIV infection. We studied the initial events associated with host-pathogen interactions by exposing cervical biopsies to free or complement-opsonized HIV. Opsonization resulted in higher rates of HIV acquisition/infection in mucosal tissues and emigrating dendritic cells. Transcriptomic and proteomic data showed a significantly more pathways and higher expression of genes and proteins associated with viral replication and pathways involved in different aspects of viral infection including interferon signaling, cytokine profile and dendritic cell maturation for the opsonized HIV. Moreover, the proteomics data indicate a general suppression by the HIV exposure. This clearly suggests that HIV opsonization alters the initial signaling pathways in the cervical mucosa in a manner that promotes viral establishment and infection. Our findings provide a foundation for further studies of the role these early HIV induced events play in HIV pathogenesis.