2021 journal article

An optimized protocol for stepwise optimization of real-time RT-PCR analysis


By: F. Zhao*, N. Maren n, P. Kosentka n, Y. Liao n, H. Lu n, J. Duduit n, D. Huang n, H. Ashrafi n ...

TL;DR: This work proposed an optimized approach to sequentially optimizing primer sequences, annealing temperatures, primer concentrations, and cDNA concentration range for each reference (and target) gene and validated their utility under varying abiotic stress conditions. (via Semantic Scholar)
Source: Web Of Science
Added: August 23, 2021