CRISPR-Cas9 editing of CAFFEOYL SHIKIMATE ESTERASE 1 and 2 shows their importance and partial redundancy in lignification in Populus tremula x P. alba
Vries, L., Brouckaert, M., Chanoca, A., Kim, H., Regner, M. R., Timokhin, V. I., … Boerjan, W. (2021, August 6). PLANT BIOTECHNOLOGY JOURNAL .
By: L. Vries * , M. Brouckaert * , A. Chanoca * , H. Kim * , M. Regner* , V. Timokhin* , Y. Sun * , B. De Meester * ...and 8 other authors, including 2 NC State authors , J. Van Doorsselaere* , G. Goeminne * , V. Chiang n , J. Wang n , J. Ralph * , K. Morreel * , R. Vanholme * , W. Boerjan *
author keywords: poplar; CRISPR-Cas9; CSE; lignin; phenylpropanoids; metabolic engineering
MeSH headings : CRISPR-Cas Systems / genetics; Carboxylesterase; Gene Expression Regulation, Plant; Lignin / metabolism; Plants, Genetically Modified / genetics; Plants, Genetically Modified / metabolism; Populus / genetics; Populus / metabolism
TL;DR:
The results suggest an alternative path within the phenylpropanoid pathway at the level of the hydroxycinnamoyl‐shikimates, and show that CSE is a promising target to improve plants for the biorefinery.
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