2021 article
Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests
PROTEOMIC PROFILING, 2 EDITION, Vol. 2261, pp. 489–506.
author keywords: Proteomics; Mass spectrometry; Host cell proteins; Monoclonal antibodies; Peptide ligands; Flow-through chromatography
MeSH headings : Animals; CHO Cells; Cricetulus; Enzyme-Linked Immunosorbent Assay; Humans; Ligands; Microscopy, Fluorescence; Peptide Library; Peptides / metabolism; Protein Binding; Proteins / isolation & purification; Proteins / metabolism; Proteomics; Solid Phase Extraction; Tandem Mass Spectrometry
TL;DR:
This work describes the development of HCP-binding peptide ligands, especially focusing on the steps of peptide selection via library screening and quantification of H CP removal via proteomics by mass spectrometry.
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2. Zero Hunger
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3. Good Health and Well-being
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Added: September 20, 2021
Capture of host cell proteins (HCPs) from cell culture production harvests is critical to ensure the maximum levels specified by international regulatory bodies of product purity for therapeutic monoclonal antibodies (mAbs). Peptide ligands that selectively target the whole spectrum of the HCPs, while letting the mAb product flow through unbound, are an ideal complement to the affinity-based capture step via Protein A chromatography. In this work, we describe the development of HCP-binding peptide ligands, especially focusing on the steps of (1) peptide selection via library screening and (2) quantification of HCP removal via proteomics by mass spectrometry.