Expression of a constitutively active nitrate reductase variant in tobacco reduces tobacco-specific nitrosamine accumulation in cured leaves and cigarette smoke
PLANT BIOTECHNOLOGY JOURNAL, 14(7), 1500–1510.
author keywords: nitrate reductase; tobacco; tobacco-specific nitrosamines; burley; nitrogen-assimilation pathway; NNN; NNK
MeSH headings : Carcinogens / metabolism; Metabolic Networks and Pathways; Nitrate Reductase / genetics; Nitrates / metabolism; Nitrogen / metabolism; Nitrosamines / metabolism; Plant Leaves / genetics; Plant Leaves / metabolism; Plants, Genetically Modified / metabolism; Tobacco / genetics; Tobacco / metabolism; Tobacco Products
TL;DR:
Investigation of the potential of molecular genetic strategies to lower nitrate levels in burley tobaccos by overexpressing genes encoding key enzymes of the nitrogen‐assimilation pathway found only the expression of a constitutively active nitrate reductase (NR) dramatically decreased free nitrates levels in the leaves.
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