2022 journal article

E3 ligase BRUTUS Is a Negative Regulator for the Cellular Energy Level and the Expression of Energy Metabolism-Related Genes Encoded by Two Organellar Genomes in Leaf Tissues

Molecules and Cells.

By: B. Choi *, D. Hyeon *, J. Lee *, T. Long n , D. Hwang * & I. Hwang *

author keywords: Arabidopsis thaliana; BRUTUS; energy metabolism; shoot tissues
MeSH headings : Adenosine Diphosphate / metabolism; Adenosine Triphosphate / metabolism; Arabidopsis / metabolism; Arabidopsis Proteins / metabolism; Energy Metabolism / genetics; Gene Expression Regulation, Plant; Plant Leaves / genetics; Plant Leaves / metabolism; Plant Roots / genetics; Plant Roots / metabolism; Plant Shoots; Starch / metabolism; Ubiquitin-Protein Ligases / metabolism
TL;DR: The role of BTS is expanded to regulation of energy metabolism in the shoot in addition to its role of iron deficiency response in roots through Comparative transcriptome analysis with shoot and root tissues. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (Web of Science)
7. Affordable and Clean Energy (OpenAlex)
Source: ORCID
Added: April 20, 2022

E3 ligase BRUTUS (BTS), a putative iron sensor, is expressed in both root and shoot tissues in seedlings of Arabidopsis thaliana. The role of BTS in root tissues has been well established. However, its role in shoot tissues has been scarcely studied. Comparative transcriptome analysis with shoot and root tissues revealed that BTS is involved in regulating energy metabolism by modulating expression of mitochondrial and chloroplast genes in shoot tissues. Moreover, in shoot tissues of bts-1 plants, levels of ADP and ATP and the ratio of ADP/ATP were greatly increased with a concomitant decrease in levels of soluble sugar and starch. The decreased starch level in bts-1 shoot tissues was restored to the level of shoot tissues of wild-type plants upon vanadate treatment. Through this study, we expand the role of BTS to regulation of energy metabolism in the shoot in addition to its role of iron deficiency response in roots.