2022 journal article

Triptorelin-functionalized PEG-coated biosynthesized gold nanoparticles: Effects of receptor-ligand interactions on adhesion to triple negative breast cancer cells

Biomaterials Advances, 4, 212801.

By: V. Uzonwanne*, A. Navabi*, J. Obayemi *, J. Hu* , A. Salifu*, S. Ghahremani*, N. Ndahiro*, N. Rahbar *, W. Soboyejo *

co-author countries: United States of America πŸ‡ΊπŸ‡Έ
author keywords: Adhesion; PEG-coated gold nanoparticles; Molecular dynamic simulation; Triptorelin; AFM; Triple negative breast cancer
MeSH headings : Cell Line, Tumor; Gold / pharmacology; Humans; Ligands; Metal Nanoparticles; Triple Negative Breast Neoplasms / drug therapy; Triptorelin Pamoate / pharmacology
Source: ORCID
Added: August 10, 2022

This paper presents the results of an experimental and computational study of the adhesion of triptorelin-conjugated PEG-coated biosynthesized gold nanoparticles (GNP-PEG-TRP) to triple-negative breast cancer (TNBC) cells. The adhesion is studied at the nanoscale using a combination of atomic force microscopy (AFM) experiments and molecular dynamics (MD) simulations. The AFM measurements showed that the triptorelin-functionalized gold nanoparticles (GNP-TRP and GNP-PEG-TRP) have higher adhesion to triple-negative breast cancer cells (TNBC) than non-tumorigenic breast cells. The increased adhesion of GNP-TRP and GNP-PEG-TRP to TNBC is also attributed to the overexpression of LHRH receptors on the surfaces of both TNBC. Finally, the molecular dynamics model reveals insights into the effects of receptor density, molecular configuration, and receptor-ligand docking characteristics on the interactions of triptorelin-functionalized PEG-coated gold nanoparticles with TNBC. A three to nine-fold increase in the adhesion is predicted between triptorelin-functionalized PEG-coated gold nanoparticles and TNBC cells. The implications of the results are then discussed for the specific targeting of TNBC.