2022 journal article

Carotenoids in orange carrots mitigate non-alcoholic fatty liver disease progression

FRONTIERS IN NUTRITION, 9.

By: E. Balbuena n, J. Cheng n & A. Eroglu n

author keywords: phytochemicals; nutrition; beta-oxidation; nuclear receptors; lipid metabolism
TL;DR: Carotenoids in the orange carrots reduced HFD-induced weight gain, better than white carrots, and may display more potent efficacy in mitigating NAFLD than those with low carotenoid levels. (via Semantic Scholar)
Source: Web Of Science
Added: October 24, 2022

BackgroundCarotenoids are abundant in colored fruits and vegetables. Non-alcoholic fatty liver disease (NAFLD) is a global burden and risk factor for end-stage hepatic diseases. This study aims to compare the anti-NAFLD efficacy between carotenoid-rich and carotenoid-deficient vegetables.Materials and methodsMale C57BL/6J mice were randomized to one of four experimental diets for 15 weeks (n = 12 animals/group): Low-fat diet (LFD, 10% calories from fat), high-fat diet (HFD, 60% calories from fat), HFD with 20% white carrot powders (HFD + WC), or with 20% orange carrot powders (HFD + OC).ResultsWe observed that carotenoids in the orange carrots reduced HFD-induced weight gain, better than white carrots. Histological and triglyceride (TG) analyses revealed significantly decreased HFD-induced hepatic lipid deposition and TG content in the HFD + WC group, which was further reduced in the HFD + OC group. Western blot analysis demonstrated inconsistent changes of fatty acid synthesis-related proteins but significantly improved ACOX-1 and CPT-II, indicating that orange carrot carotenoids had the potential to inhibit NAFLD by improving β-oxidation. Further investigation showed significantly higher mRNA and protein levels of PPARα and its transcription factor activity.ConclusionCarotenoid-rich foods may display more potent efficacy in mitigating NAFLD than those with low carotenoid levels.