2022 article

High-throughput quantitative binding analysis of DNA aptamers using exonucleases

Canoura, J., Alkhamis, O., Liu, Y., Willis, C., & Xiao, Y. (2022, December 30). NUCLEIC ACIDS RESEARCH.

By: J. Canoura n, O. Alkhamis n, Y. Liu n, C. Willis n & Y. Xiao n 

MeSH headings : Aptamers, Nucleotide / chemical synthesis; Aptamers, Nucleotide / chemistry; Exonucleases; Ligands; Nucleic Acids; SELEX Aptamer Technique / methods; Fentanyl / analysis; Robotics
TL;DR: This work has developed a high-throughput method for accurately quantifying aptamer binding affinity, specificity, and cross-reactivity via the kinetics of aptamer digestion by exonucleases and this approach dramatically accelerates the aptamer characterization process and streamlines sensor development. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Source: Web Of Science
Added: January 17, 2023

Abstract