2022 article

The double flower variant of yellowhorn is due to a LINE1 transposon-mediated insertion

Wang, H., Lu, Y., Zhang, T., Liu, Z., Cao, L., Chang, Q., … Zheng, Z. (2022, December 10). PLANT PHYSIOLOGY.

By: H. Wang *, Y. Lu *, T. Zhang *, Z. Liu *, L. Cao*, Q. Chang *, Y. Liu *, X. Lu * ...

MeSH headings : Phenotype; Sapindaceae / genetics; Magnoliopsida / genetics; DNA Transposable Elements / genetics; Flowers / genetics; Gene Expression Regulation, Plant
TL;DR: This work found a candidate C-class gene, AGAMOUS1 (XsAG1), through BSA sequencing and genetics analysis with a LONG INTERSPERSED NUCLEAR ELEMENTS 1 (LINE1) transposable element fragment inserted into its second intron that caused a loss-of-C-function and therefore the double-flower phenotype in yellowhorn. (via Semantic Scholar)
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2. Zero Hunger (Web of Science)
13. Climate Action (Web of Science)
15. Life on Land (OpenAlex)
Source: Web Of Science
Added: February 20, 2023

AbstractAs essential organs of reproduction in angiosperms, flowers, and the genetic mechanisms of their development have been well characterized in many plant species but not in the woody tree yellowhorn (Xanthoceras sorbifolium). Here, we focused on the double flower phenotype in yellowhorn, which has high ornamental value. We found a candidate C-class gene, AGAMOUS1 (XsAG1), through Bulked Segregant Analysis (BSA) sequencing and genetics analysis with a Long Interpersed Nuclear Elements 1 (LINE1) transposable element fragment (Xsag1-LINE1-1) inserted into its second intron that caused a loss-of-C-function and therefore the double flower phenotype. In situ hybridization of XsAG1 and analysis of the expression levels of other ABC genes were used to identify differences between single- and double-flower development processes. These findings enrich our understanding of double flower formation in yellowhorn and provide evidence that transposon insertions into genes can reshape plant traits in forest trees.