@article{nole-wilson_rueschhoff_bhatti_franks_2010, title={Synergistic disruptions in seuss cyp85A2 double mutants reveal a role for brassinolide synthesis during gynoecium and ovule development}, volume={10}, ISSN={["1471-2229"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-77956486883&partnerID=MN8TOARS}, DOI={10.1186/1471-2229-10-198}, abstractNote={Abstract
          
            Background
            The Arabidopsis SEUSS (SEU) gene encodes a transcriptional adaptor protein that is required for a diverse set of developmental events, including floral organ identity specification, as well as gynoecium, ovule and embryo development. In order to better understand the molecular mechanisms of SEUSS action we undertook a genetic modifier screen to identify seuss-modifier (sum) mutations.
          
          
            Results
            Screening of M2 lines representing approximately 5,000 M1 individuals identified mutations that enhance the seuss mutant phenotypic disruptions in ovules and gynoecia; here we describe the phenotype of the sum63 mutant and enhanced disruptions of ovule and gynoecial development in the seu sum63 double mutant. Mapping and genetic complementation tests indicate that sum63 is allelic to CYP85A2 (AT3G30180) a cytochrome p450 enzyme that catalyzes the final steps in the synthesis of the phytohormone brassinolide.
          
          
            Conclusions
            Our identification of mutations in CYP85A2 as enhancers of the seuss mutant phenotype suggests a previously unrecognized role for brassinolide synthesis in gynoecial and ovule outer integument development. The work also suggests that seuss mutants may be more sensitive to the loss or reduction of brassinolide synthesis than are wild type plants.
          }, journal={BMC PLANT BIOLOGY}, author={Nole-Wilson, Staci and Rueschhoff, Elizabeth E. and Bhatti, Huda and Franks, Robert G.}, year={2010}, month={Sep} }
 @article{azhakanandam_nole-wilson_bao_franks_2008, title={SEUSS and AINTEGUMENTA mediate patterning and ovule initiation during gynoecium medial domain development}, volume={146}, ISSN={["0032-0889"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-48949120198&partnerID=MN8TOARS}, DOI={10.1104/pp.107.114751}, abstractNote={AbstractThe Arabidopsis (Arabidopsis thaliana) gynoecium, the female floral reproductive structure, requires the action of genes that specify positional identities during its development to generate an organ competent for seed development and dispersal. Early in gynoecial development, patterning events divide the primordium into distinct domains that will give rise to specific tissues and organs. The medial domain of the gynoecium gives rise to the ovules, and several other structures critical for reproductive competence. Here we report a synergistic genetic interaction between seuss and aintegumenta mutants resulting in a complete loss of ovule initiation and a reduction of the structures derived from the medial domain. We show that patterning events are disrupted early in the development of the seuss aintegumenta gynoecia and we identify PHABULOSA (PHB), REVOLUTA, and CRABS CLAW (CRC) as potential downstream targets of SEUSS (SEU) and AINTEGUMENTA (ANT) regulation. Our genetic data suggest that SEU additionally functions in pathways that are partially redundant and parallel to PHB, CRC, and ANT. Thus, SEU and ANT are part of a complex and robust molecular system that coordinates patterning cues and cellular proliferation along the three positional axes of the developing gynoecium.}, number={3}, journal={PLANT PHYSIOLOGY}, author={Azhakanandam, Sridevi and Nole-Wilson, Staci and Bao, Fang and Franks, Robert G.}, year={2008}, month={Mar}, pages={1165–1181} }