@article{walker_klaenhammer_2001, title={Leaky Lactococcus cultures that externalize enzymes and antigens independently of culture lysis and secretion and export pathways}, volume={67}, ISSN={["1098-5336"]}, DOI={10.1128/AEM.67.1.251-259.2001}, abstractNote={ABSTRACT}, number={1}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Walker, SA and Klaenhammer, TR}, year={2001}, month={Jan}, pages={251–259} } @article{walker_klaenhammer_2000, title={An explosive antisense RNA strategy for inhibition of a lactococcal bacteriophage}, volume={66}, ISSN={["0099-2240"]}, DOI={10.1128/AEM.66.1.310-319.2000}, abstractNote={ABSTRACT}, number={1}, journal={APPLIED AND ENVIRONMENTAL MICROBIOLOGY}, author={Walker, SA and Klaenhammer, TR}, year={2000}, month={Jan}, pages={310–319} } @misc{klaenhammer_conkling_o'sullivan_djordjevic_walker_taylor_1998, title={Bacteriophage-triggered cell suicide systems and fermentation methods employing the same}, volume={5,792,625}, number={1998 Aug. 11}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Klaenhammer, T. R. and Conkling, M. A. and O'Sullivan, D. and Djordjevic, G. and Walker, S. A. and Taylor, C. G.}, year={1998} } @article{walker_dombroski_klaenhammer_1998, title={Common elements regulating gene expression in temperate and lytic bacteriophages of Lactococcus species}, volume={64}, number={3}, journal={Applied and Environmental Microbiology}, author={Walker, S. A. and Dombroski, C. S. and Klaenhammer, T. R.}, year={1998}, pages={1147–1152} } @article{walker_klaenhammer_1998, title={Molecular characterization of a phage-inducible middle promoter and its transcriptional activator from the lactococcal bacteriophage phi 31}, volume={180}, number={4}, journal={Journal of Bacteriology}, author={Walker, S. A. and Klaenhammer, T. R.}, year={1998}, pages={921–931} } @article{djordjevic_osullivan_walker_conkling_klaenhammer_1997, title={A triggered-suicide system designed as a defense against bacteriophages}, volume={179}, ISSN={["0021-9193"]}, DOI={10.1128/jb.179.21.6741-6748.1997}, abstractNote={A novel bacteriophage protection system for Lactococcus lactis based on a genetic trap, in which a strictly phage-inducible promoter isolated from the lytic phage phi31 is used to activate a bacterial suicide system after infection, was developed. The lethal gene of the suicide system consists of the three-gene restriction cassette LlaIR+, which is lethal across a wide range of gram-positive bacteria. The phage-inducible trigger promoter (phi31P) and the LlaIR+ restriction cassette were cloned in Escherichia coli on a high-copy-number replicon to generate pTRK414H. Restriction activity was not apparent in E. coli or L. lactis prior to phage infection. In phage challenges of L. lactis(pTRK414H) with phi31, the efficiency of plaquing was lowered to 10(-4) and accompanied by a fourfold reduction in burst size. Center-of-infection assays revealed that only 15% of infected cells released progeny phage. In addition to phage phi31, the phi31P/LlaIR+ suicide cassette also inhibited four phi31-derived recombinant phages at levels at least 10-fold greater than that of phi31. The phi31P/LlaIR+-based suicide system is a genetically engineered form of abortive infection that traps and eliminates phages potentially evolving in fermentation environments by destroying the phage genome and killing the propagation host. This type of phage-triggered suicide system could be designed for any bacterium-phage combination, given a universal lethal gene and an inducible promoter which is triggered by the infecting bacteriophage.}, number={21}, journal={JOURNAL OF BACTERIOLOGY}, author={Djordjevic, GM and OSullivan, DJ and Walker, SA and Conkling, MA and Klaenhammer, TR}, year={1997}, month={Nov}, pages={6741–6748} } @misc{vandenbergh_walker_kunkar_1997, title={Method of making a lactococcal bacteriocin}, volume={5,702,923}, number={1997 Dec. 30}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Vandenbergh, P. A. and Walker, S. A. and Kunkar, B. S.}, year={1997} }