@article{fellers_tremblay_handest_lommel_2002, title={The Potato virus Y (MNR)-N-S Nlb-replicase is the elicitor of a veinal necrosis-hypersensitive response in root knot nematode resistant tobacco}, volume={3}, ISSN={["1464-6722"]}, DOI={10.1046/j.1364-3703.2002.00106.x}, abstractNote={Summary}, number={3}, journal={MOLECULAR PLANT PATHOLOGY}, author={Fellers, JP and Tremblay, D and Handest, MF and Lommel, SA}, year={2002}, month={May}, pages={145–152} } @article{fellers_collins_hunt_1998, title={The NIa-proteinase of different plant potyviruses provides specific resistance to viral infection}, volume={38}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1998.0011183X003800050030x}, abstractNote={The genome‐linked protein‐proteinase (NIa) genes of three potyviruses (tobacco vein mottling virus[TVMV], tobacco etch virus[TEV], and potato virus Y [PVY]) were introduced into tobacco (Nicotiana tabacum L.) cultivar Burley 21 and transgenic plant lines were evaluated for viral symptoms and virus accumulation. Plant lines that expressed the TVMV NIa gene were resistant to infection by TVMV; inoculated plants did not develop disease symptoms after inoculation, and virus did not accumulate. Most lines that carried the TEV NIa and PVY NIa genes did develop symptoms after inoculation with TEV and PVY, respectively, but these symptoms abated with time (a response termed as recovery). Three genes, each consisting of two NIa coding regions (TEV NIa‐PVY NIa, TEV NIa‐TVMV NIa, and TVMV NIa‐PVY NIa) were also introduced into Burley 21. Transgenic lines that carried either TEV NIa‐PVY NIa or TEV NIa‐TVMV NIa constructs either did not develop disease symptoms or exhibited the process of recovery after inoculation with TEV. TEV NIa‐TVMV NIa and TVMV NIa‐PVY NIa lines recovered from an initial infection after inoculation with TVMVL. Likewise, TEV NIa‐PVY NIa and TVMV NIa‐PVY NIa transformed lines recovered from an initial infection after inoculation with PVY. These results demonstrate that different potyvirus NIa‐proteinase genes can be used for protection against virus infection and that multiple NIa genes may be used to produce plants that are protected against more than one potyvirus.}, number={5}, journal={CROP SCIENCE}, author={Fellers, JP and Collins, GB and Hunt, AG}, year={1998}, pages={1309–1319} } @article{fellers_guenzi_porter_1997, title={Marker proteins associated with somatic embryogenesis of wheat callus cultures}, volume={151}, ISSN={["0176-1617"]}, DOI={10.1016/S0176-1617(97)80153-8}, abstractNote={Summary A marker protein for embryogenic potential could be useful in determining if target tissue for microprojectile bombardment has the ability to regenerate plants. The identification of such a protein in wheat callus cultures was approached by using isoelectric focusing and SDS-PAGE of proteins labeled in vivo with 35[S]-methionine and cysteine. Protein profile differences were examined in embryogenic (E-callus) and non-embryogenic (NE-callus) wheat callus, 105 or 271 d after callus initiation. Callus was maintained on medium containing either 5.6 or 9 μmol/L 2,4-dichlorophenoxyacetic acid (2,4-D). Proteins unique to E-callus (E-proteins) were identified by computer assisted analysis of scanned images of fluorographs of in vivo-labeled proteins of E- and NE-callus. Thirty-three E-proteins were identified in 105-day-old E-callus growing on 5.6 μmol/L 2,4-D, 71 E-proteins in 105-day-old callus on 9 μmol/L 2,4-D, 43 E-proteins in 271-day-old callus on 5.6 μmol/L 2,4-D, and 39 E-proteins in 271-day-old callus growing on 9 μmol/L 2,4-D. Of these E-proteins, 10 were in 105-day-old callus regardless of 2,4-D concentration. One E-protein was present in 271-day-old callus from both 2,4-D concentrations. Two E-proteins with relative molecular masses/pis of 43.0/7.6 and 27.0/8.2 were present in E-callus from three of the four treatments. These proteins could be used as markers for determining if tissue has embryogenic potential.}, number={2}, journal={JOURNAL OF PLANT PHYSIOLOGY}, author={Fellers, JP and Guenzi, AC and Porter, DR}, year={1997}, month={Aug}, pages={201–208} }