@article{huang_dauterman_hastings_1974, title={INHIBITION OF FLYHEAD ACETYLCHOLINESTERASE BY DIMETHOXON ANALOGS}, volume={4}, ISSN={["1095-9939"]}, DOI={10.1016/0048-3575(74)90107-2}, abstractNote={Abstract The inhibition of purified housefly-head acetylcholinesterase by 16 dimethoxon analogs was studied and the phosphorylation and affinity constants determined. Except for compound XV, all the analogs were better inhibitors of flyhead acetylcholinesterase than of bovine erythrocyte acetylcholinesterase. The large bimolecular reaction constants were the result of higher affinity and phosphorylation rates. Mono-substitution on the carbamoyl nitrogen primarily affected reactivity (k2), whereas di-substitution affected affinity (Ka). Modification of the 0,0-dialkyl moiety had little effect on affinity although reactivity decreased proportionately with chain length. There is some indication that the interaction of these inhibitors with flyhead AChE is due to a considerable electrostatic contribution. The difference between target enzymes (housefly versus erythrocyte AChE) accounts in part for the selectivity of dimethoate and its analogs.}, number={3}, journal={PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY}, author={HUANG, CT and DAUTERMAN, WC and HASTINGS, FL}, year={1974}, pages={249–253} }
 @article{huang_dauterman_1973, title={PURIFICATION OF FLY HEAD CHOLINESTERASE}, volume={3}, ISSN={["0020-1790"]}, DOI={10.1016/0020-1790(73)90064-4}, abstractNote={A cholinesterase from the housefly head was purified by butanol solubilization, ammonium sulphate, and acetone fractionations and Sephadex G-200, DEAE-cellulose, Sepharose 6-B, and DEAE-Sephadex A-50 gel chromatography. The enzyme was purified about 1300 times and had a specific activity of 739 (units per mg.) Its molecular weight was estimated to be 209,000 by Sepharose 6-B gel filtration. With acetylthiocholine, aKm of 0.014 mM and a turnover number of 123,800 was obtained at 25°C. and pH 8.0. Disc electrophoresis revealed that two interconvertible enzymatic forms existed in the purified enzyme.}, number={11}, journal={INSECT BIOCHEMISTRY}, author={HUANG, CT and DAUTERMAN, WC}, year={1973}, pages={325–334} }