@article{kramer_reed_1988, title={AN EVALUATION OF MATERNAL NULLIHAPLOIDY FOR NICOTIANA-TABACUM-L NULLISOMIC PRODUCTION .1. AN INTERSPECIFIC HYBRIDIZATION APPROACH}, volume={79}, ISSN={["0022-1503"]}, DOI={10.1093/oxfordjournals.jhered.a110439}, abstractNote={The value of a maternal nullihaploidy system for the production of Nicotiana tabacum L. nullisomics was investigated. Five monosmics of the Purpurea genetic background, F, L, M, P, and R, and three of the SC 58 background, C, F, and P, were crossed as females to Nicotiana africana Merxm. Over 99% of the progeny of these crosses were lethal interspecific hybrids, which, as expected, died at the cotyledonary stage. The majority of the surviving plants were aneuploid hybrids, having chromosome numbers ranging from 25 to 90. A few maternal haploids and one paternal haploid were also obtained, but no nullihaploids were isolated. The failure of this maternal haploidy system to produce nullihaploids may have been because of a low rate of induced gynogenesis, seeding conditions that were inadequate to sustain nullihaploid development, and the natural inviability of a nullihaploid plant type. Until these factors are minimized or eliminated, the utility of this interspecific hybridization approach for nullisomic production may be negligible.}, number={1}, journal={JOURNAL OF HEREDITY}, author={KRAMER, MG and REED, SM}, year={1988}, pages={24–27} } @article{kramer_reed_1988, title={AN EVALUATION OF MATERNAL NULLIHAPLOIDY FOR NICOTIANA-TABACUM-L NULLISOMIC PRODUCTION .2. A POLLEN IRRADIATION AND OVULE CULTURE APPROACH}, volume={79}, ISSN={["0022-1503"]}, DOI={10.1093/oxfordjournals.jhered.a110553}, abstractNote={Production de nullihaploides chez N. tabacum realisee par la procedure d'haploidie maternelle induite par irradiation du pollen et par culture d'ovule}, number={6}, journal={JOURNAL OF HEREDITY}, author={KRAMER, MG and REED, SM}, year={1988}, pages={469–472} }