@inbook{hessler_whetten_loopstra_penner_shriver_zeigler_fletcher_torrie_comstock_2002, title={Golden rice}, ISBN={081382835X}, booktitle={Life science ethics}, publisher={Ames, IA: Iowa State University Press}, author={Hessler, K. and Whetten, R. and Loopstra, C. and Penner, K. P. and Shriver, S. and Zeigler, R. and Fletcher, J. and Torrie, M. and Comstock, G. L.}, year={2002}, pages={307–368} } @article{loopstra_sederoff_1995, title={XYLEM-SPECIFIC GENE-EXPRESSION IN LOBLOLLY-PINE}, volume={27}, ISSN={["0167-4412"]}, DOI={10.1007/BF00020183}, abstractNote={Two genes preferentially expressed in differentiating xylem of loblolly pine (Pinus taeda L.) were cloned from cDNA and genomic libraries and designated PtX3H6 and PtX14A9. Transcripts of PtX3H6 and PtX14A9 are very abundant in differentiating xylem, less abundant in needles, and very low or non-detectable in embryos and megagametophytes. PtX3H6 contains a putative signal peptide, a threonine-rich region, a proline-rich region, and a hydrophobic tail. Repeats of Pro-Pro-Pro-Val-X-X are similar to repeats found in proline-rich cell wall proteins. The amino acid compositions of PtX3H6 and PtX14A9 are similar to those of arabinogalactan proteins (AGPs). PtX14A9 contains an 8 amino acid sequence similar to amino terminal sequences of ryegrass, carrot and rose AGPs. Upstream sequences have been determined from genomic clones encoding PtX3H6 and PtX14A9. A 7 bp sequence found in the 5' flanking regions of both genes has previously been shown to be involved in the vascular-specific expression of GRP 1.8, a glycine-rich protein found in bean. The sequence is also present upstream of another glycine-rich protein from bean, GRP 1.0, and may be partially responsible for the xylem-specific expression of pTx3H6 and PtX14A9.}, number={2}, journal={PLANT MOLECULAR BIOLOGY}, author={LOOPSTRA, CA and SEDEROFF, RR}, year={1995}, month={Jan}, pages={277–291} } @article{loopstra_weissinger_sederoff_1992, title={TRANSIENT GENE-EXPRESSION IN DIFFERENTIATING PINE WOOD USING MICROPROJECTILE BOMBARDMENT}, volume={22}, ISSN={["0045-5067"]}, DOI={10.1139/x92-133}, abstractNote={ We have used microprojectile bombardment to obtain transient expression of the reporter gene β-glucuronidase in differentiating wood (secondary xylem) of loblolly pine (Pinustaeda L.), thereby providing a method for studying expression of introduced DNA in this important tissue. β-Glucuronidase activity can be observed in different cell types, including tracheids, ray parenchyma, and axial parenchyma associated with resin canals. Microprojectile bombardment can be used to identify active promoters and to compare the relative activities of different promoters in specific cell types. We have studied the expression of three promoter gene fusions by bombardment of loblolly pine stem sections. Wood samples bombarded with an abscisic acid inducible (Em) promoter–β-glucuronidase fusion contained nearly 10 times the number of stained cells as those bombarded with two more commonly used promoter fusions (nopaline synthase or cauliflower mosaic virus 35S). Microprojectile bombardment of differentiating wood should be useful for studying promoters and gene constructs in future attempts to modify wood properties by genetic engineering. }, number={7}, journal={CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE}, author={LOOPSTRA, CA and WEISSINGER, AK and SEDEROFF, RR}, year={1992}, month={Jul}, pages={993–996} }