@article{faith_kathariou_cheng_promadej_neudeck_zhang_luchansky_czuprynski_2009, title={The Role of L-monocytogenes Serotype 4b gtcA in Gastrointestinal Listeriosis in A/J Mice}, volume={6}, ISSN={["1556-7125"]}, DOI={10.1089/fpd.2008.0154}, abstractNote={Serotype 4b strains of Listeria monocytogenes have been responsible for most large outbreaks of listeriosis. In L. monocytogenes serotype 4b, gtcA and gltA have been implicated in serotype-specific glycosylation of the teichoic acid of the cell wall with galactose and glucose. In this study, we investigated the impact of mutations in gltA (resulting in absence of glucose on teichoic acid) and gtcA (resulting in absence of galactose, and markedly reduced glucose on teichoic acid) on virulence following intragastric infection of anesthetized A/J mice. The gltA mutant was not impaired in virulence in this model. In contrast, testing of gtcA mutants constructed in two different strains showed that the mutants were recovered in lower numbers than their respective parent strains from the spleen, liver, ceca, and gall bladders of intragastrically inoculated mice. Genetic complementation of the gtcA mutation partially restored gastrointestinal virulence. When mice were inoculated intravenously, the gtcA mutants were also recovered in lower numbers from the liver (for both mutant strains) and the spleen (for one mutant strain) than their respective parental strains. The mutants were also evaluated for invasion and intracellular multiplication in the Caco-2 human intestinal epithelial cell line. Inactivation of gltA did not affect invasion or intracellular growth of the bacteria. In contrast, gtcA mutants showed decreased invasion, but normal multiplication in Caco-2 cells. Overall, these data demonstrate a role for gtcA in the pathogenesis of gastrointestinal listeriosis in mice, and suggest that diminished ability of gtcA mutants to invade intestinal epithelial cells may be partly responsible for decreased gastrointestinal virulence.}, number={1}, journal={FOODBORNE PATHOGENS AND DISEASE}, author={Faith, Nancy and Kathariou, Sophia and Cheng, Ying and Promadej, Nattawan and Neudeck, Brien L. and Zhang, Qiuye and Luchansky, John and Czuprynski, Charles}, year={2009}, pages={39–48} }
 @article{luchansky_tennant_klaenhammer_1991, title={MOLECULAR-CLONING AND DEOXYRIBONUCLEIC-ACID POLYMORPHISMS IN LACTOBACILLUS-ACIDOPHILUS AND LACTOBACILLUS-GASSERI}, volume={74}, ISSN={["0022-0302"]}, DOI={10.3168/jds.S0022-0302(91)78515-9}, abstractNote={Lactobacillus strain ADH is a bile-resistant, bacteriocin-producing human isolate that was phenotypically classified within the Lactobacillus acidophilus group. Total DNA and phage DNA extracted from strain ADH were separately digested with BclI and ligated with BclI-digested pGK12. Following electroporation of these ligation mixtures directly into strain ADH, electrotransformants were recovered at frequencies of 1.5 x 10(3) and 2.0 x 10(4)/micrograms of pGK12 for preparations of pGK12::phage DNA and pGK12::total DNA, respectively. Among the electrotransformants screened, 6 and 22% contained passenger DNA of either phage DNA or chromosomal origin, respectively, as determined by restriction-enzyme analyses and hybridization assays. Derivatives of pGK12 containing passenger DNA of chromosomal (pTRK120) or phage (pTRK121) origin and pTRK15 (native cryptic plasmid) were evaluated for use as species-specific probes. The strain ADH-derived probes hybridized primarily to members of the B-1 and B-2 lactobacilli homology groups and demonstrated strain-specific polymorphisms within these groups. Identical hybridization patterns were established for strain ADH and Lactobacillus gasseri VPI 6033 (ATCC 19992). Identification of DNA probes and establishment of a host-vector cloning system have facilitated our efforts to characterize the Lactobacillus chromosome and to distinguish between closely related species thought to be important inhabitants of the gastrointestinal tract.}, number={10}, journal={JOURNAL OF DAIRY SCIENCE}, author={LUCHANSKY, JB and TENNANT, MC and KLAENHAMMER, TR}, year={1991}, month={Oct}, pages={3293–3302} }
 @article{luchansky_tennant_raya_klaenhammer_1989, title={Identification of temperature-dependent replication of pGK12 and direct cloning via electroporation in Lactobacillus acidophilus ADH}, volume={72}, journal={Journal of Dairy Science}, author={Luchansky, J. B. and Tennant, M. C. and Raya, R. R. and Klaenhammer, T. R.}, year={1989}, pages={113} }