@article{mcgahan_grimes_nasisse_fleisher_1995, title={IRON UPTAKE BY CULTURED LENS EPITHELIAL-CELLS}, volume={233}, ISSN={["0721-832X"]}, DOI={10.1007/BF00200484}, abstractNote={{"Label"=>"BACKGROUND", "NlmCategory"=>"BACKGROUND"} Transferrin and Fe concentrations increase in the intraocular fluids in pathological conditions and the lens accumulates Fe during ocular inflammation. Tissues take up Fe from transferrin by two mechanisms, receptor-medicated endocytosis of diferric transferrin and a process occurring at the cell membrane which may be mediated by an oxido-reductase. However, Fe metabolism, transport and storage have not been previously investigated in the lens. This study was designed to characterize the uptake of Fe from transferrin by lens epithelial cells in culture. {"Label"=>"METHODS", "NlmCategory"=>"METHODS"} Primary, secondary and tertiary cultures of canine lens epithelial cells and cultures obtained from cataractous lenses were studied. Uptake of 59Fe from transferrin by these cultured cells was measured. Transferrin receptor populations were determined in receptor-binding assays. {"Label"=>"RESULTS", "NlmCategory"=>"RESULTS"} There was a distinct relationship between the amount of Fe-transferrin added and the amount of Fe taken up, which was linear for the primary cultures but significantly reduced for the secondary, tertiary and cataract cultures (252 +/- 21, 169 +/- 14, 153 +/- 14 and 96 +/- 2 ng Fe/mg protein, respectively). Transferring receptor expression in lens cell cultures was reduced 10-fold within 2 days of addition of serum to cells grown in low-Fe, serum-free medium for 1 week. {"Label"=>"CONCLUSIONS", "NlmCategory"=>"CONCLUSIONS"} The reduction of Fe uptake by the subcultured and cataract cell lines probably reflects a decrease in transferrin receptor expression and in the activity of an alternative pathway for Fe transferrin uptake occurring over time. This reduced Fe uptake may result from long-term exposure to relatively high Fe concentration in the media. A reduction in the expression of the transferrin receptor after incubation with high concentrations of Fe supports this conclusion.}, number={6}, journal={GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY}, author={MCGAHAN, MC and GRIMES, AM and NASISSE, MP and FLEISHER, LN}, year={1995}, month={Jun}, pages={354–359} }
 @article{nasisse_english_tompkins_guy_sussman_1995, title={Immunologic, histologic and virologic features of herpesvirus-induced stromal keratitis in cats}, volume={56}, number={1}, journal={American Journal of Veterinary Research}, author={Nasisse, M. P. and English, R. V. and Tompkins, M. B. and Guy, J. S. and Sussman, W.}, year={1995}, pages={51} }
 @article{echelman_nasisse_shields_mcgahan_fleisher_1994, title={INFLUENCE OF EXPOSURE TIME ON INFLAMMATORY RESPONSE TO NEODYMIUM - YAG CYCLOPHOTOCOAGULATION IN RABBITS}, volume={112}, ISSN={["1538-3601"]}, DOI={10.1001/archopht.1994.01090190125032}, abstractNote={OBJECTIVE
To evaluate the influence of duration of exposure on the inflammatory response to transscleral neodymium:YAG cyclophotocoagulation.


METHODS
Transscleral cyclophotocoagulation was performed with a contact, Nd:YAG, continuous-wave laser on one eye of 48 Dutch belted rabbits, 10 W and 0.2 second used in half and 1 W and 2 seconds in the other half. One third of each group was evaluated on the operative day and the other thirds on postoperative days 3 and 10. Tissue reaction was inspected grossly and by light microscopy, and inflammatory responses were measured by aqueous leukocyte and erythrocyte counts, aqueous and vitreous protein levels, aqueous prostaglandin levels, and iris and ciliary body myeloperoxidase activity.


RESULTS
The shorter-duration protocol was associated with more ciliary epithelial disruption and significantly greater inflammatory responses by one or more of the measures at all times.


CONCLUSION
When energy is constant, a shorter duration of exposure with transscleral Nd:YAG cyclophotocoagulation in rabbits is associated with greater tissue disruption and inflammation.}, number={7}, journal={ARCHIVES OF OPHTHALMOLOGY}, author={ECHELMAN, DA and NASISSE, MP and SHIELDS, MB and MCGAHAN, MC and FLEISHER, LN}, year={1994}, month={Jul}, pages={977–981} }
 @article{nasisse_guy_stevens_english_davidson_1993, title={Clinical and laboratory findings in chronic conjunctivitis in cats: 91 cases (1983-1991)}, volume={203}, number={6}, journal={Journal of the American Veterinary Medical Association}, author={Nasisse, M. P. and Guy, J. S. and Stevens, J. B. and English, R. V. and Davidson, M. G.}, year={1993}, pages={834} }
 @article{nasisse_davidson_olivero_brinkmann_nelms_1993, title={Neodymium:YAG laser treatment of primary canine intraocular tumors}, volume={3}, number={4}, journal={Veterinary and Comparative Ophthalmology}, author={Nasisse, M. P. and Davidson, M. G. and Olivero, D. K. and Brinkmann, D. K. and Nelms, S.}, year={1993}, pages={152} }
 @article{nasisse_davis_guy_davidson_sussman_1992, title={Isolation of Feline Herpesvirus 1 from the Trigeminal Ganglia of Acutely and Chronically Infected Cats}, volume={6}, ISSN={0891-6640 1939-1676}, url={http://dx.doi.org/10.1111/j.1939-1676.1992.tb03159.x}, DOI={10.1111/j.1939-1676.1992.tb03159.x}, abstractNote={Feline herpesvirus 1 (FHV‐1) is one of the most common viral infections of domestic cats worldwide, estimated to cause 50% of all respiratory infections in this species. Feline herpesvirus 1 is also an important ocular pathogen of cats, causing conjunctivitis, epithelial and stromal keratitis, symblepharon formation, keratocon‐junctivitis sicca, and corneal sequestration. Despite the importance of this viral disease, major questions remain unanswered concerning the pathogenesis of its most important manifestation, the recrudescent infection. Although the taxonomic classification of FHV‐1 as an alpha herpesvirus implies the ability of FHV‐1 to establish neural latency, attempts at recovering the virus from the trigeminal ganglia of latently infected cats have typically yielded negative results. This failure has stimulated speculation that neural tissue is not an important site for latent FHV‐1. However, in the most successful of such studies, FHV‐1 was isolated from the trigeminal ganglia of 3 of 17 cats using an explant technique. In the present study, we describe the successful isolation of FHV‐1 from the trigeminal ganglia of cats using a similar tissue culture method.}, number={2}, journal={Journal of Veterinary Internal Medicine}, publisher={Wiley}, author={Nasisse, Mark P. and Davis, Barbara J. and Guy, James S. and Davidson, Michael G. and Sussman, Wendy}, year={1992}, month={Mar}, pages={102–103} }
 @article{nasisse_davidson_jamieson_english_olivero_1991, title={Phacoemulsification and intraocular lens implantation: A study of technique in 182 dogs}, volume={1}, number={4}, journal={Veterinary and Comparative Ophthalmology}, author={Nasisse, M. P. and Davidson, M. G. and Jamieson, V. E. and English, R. V. and Olivero, D. K.}, year={1991}, pages={225} }
 @article{nasisse_davidson_english_roberts_newman_1990, title={Neodymium:YAG laser treatment of lens extraction-induced pupillary opacification in dogs}, volume={26}, number={3}, journal={Journal of the American Animal Hospital Association}, author={Nasisse, M. P. and Davidson, M. G. and English, R. V. and Roberts, S. M. and Newman, H. C.}, year={1990}, pages={275} }
 @article{nasisse_davidson_english_jamieson_harling_tate_1990, title={Treatment of glaucoma by use of transscleral neodymium:yttrium aluminium garnet laser cyclocoagulation in dogs}, volume={197}, number={3}, journal={Journal of the American Veterinary Medical Association}, author={Nasisse, M. P. and Davidson, M. G. and English, R. V. and Jamieson, V. and Harling, D. E. and Tate, L. P.}, year={1990}, pages={50} }