@article{rottman_tompkins_tompkins_1996, title={A reverse transcription-quantitative competitive polymerase chain reaction (RT-qcPCR) technique to measure cytokine gene expression in domestic mammals}, volume={33}, ISSN={["0300-9858"]}, DOI={10.1177/030098589603300217}, abstractNote={ Inbred strains of rats and mice have long been used to study basic mechanisms of human disease. Our knowledge of the rodent and human immune systems has increased in recent years, largely because of the availability of reagents and techniques specific for these species. In contrast, outbred animals, including domestic companion and food animals, have not been used routinely as experimental models for human disease, largely because reagents and assays necessary for basic research in immunology and physiology have not been available. Here, using consensus cytokine nucleic acid sequences, we adapt a previously described reverse transcription-quantitative competitive polymerase chain reaction technique to measure interleukin 2 (IL2), IL4, IL6, IL10, IL12, interferon γ, tumor necrosis factor α, and glyceraldehyde-3-phosphate dehydrogenase mRNA expression in the cow, cat, dog, horse, and pig. We demonstrate that the assay is sensitive, accurate, and reproducible. }, number={2}, journal={VETERINARY PATHOLOGY}, author={Rottman, JB and Tompkins, WAF and Tompkins, MB}, year={1996}, month={Mar}, pages={242–248} }
 @article{rottman_freeman_tonkonogy_tompkins_1995, title={A REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION TECHNIQUE TO DETECT FELINE CYTOKINE GENES}, volume={45}, ISSN={["0165-2427"]}, DOI={10.1016/0165-2427(94)05324-L}, abstractNote={The ability to detect feline cytokine expression would allow further characterization of the feline immune system. Bioassays are currently available for the measurement of feline IL2, IL6 and TNFα but not for other biologically important cytokines. To detect the expression of other cytokines, a reverse transcription-polymerase chain reaction (RT-PCR) procedure was developed. Since feline cytokine gene sequences other than TNFα were not available, mammalian DNA and mRNA sequences for IL2, IFNγ, IL4, IL6, IL10, IL12 and β-actin, obtained from the Genbank database were compared and oligonucleotide primers chosen from consensus sequences. To validate the cytokine and β-actin primers, peripheral blood mononuclear cells from specific pathogen free (SPF) cats were cultured in the presence of Con A for various periods of time (0–72 h). RNA was collected, reverse transcribed into cDNA, and the cDNA was amplified by PCR with each set of cytokine primer pairs. RT-PCR products were hybridized with specific 32P end-labeled internal oligonucleotide probes and then analyzed with the AMBIS imaging system to determine the kinetics of cytokine mRNA production. The β-actin signal was used to control for sample to sample variation in the quantity of mRNA and variation in the RT and PCR reactions. Peak mRNA expression for most cytokines was found to occur between 2 to 4 h of Con A stimulation. mRNA expression was correlated with cytokine bioactivity for IL2 and IL6. Peak IL2 bioactivity occurred after 8 h of Con A stimulation, 4 h after the mRNA expression had peaked. Although IL6 mRNA expression peaked between 2 and 4 h of stimulation, bioactivity was not detected until 8 h of stimulation and continued to increase over the next 24–48 h.}, number={1-2}, journal={VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}, author={ROTTMAN, JB and FREEMAN, EB and TONKONOGY, S and TOMPKINS, MB}, year={1995}, month={Mar}, pages={1–18} }
 @article{rottman_english_breitschwerdt_duncan_1991, title={Bone marrow hypoplasia in a cat treated with griseofulvin}, volume={198}, number={3}, journal={Journal of the American Veterinary Medical Association}, author={Rottman, J. B. and English, R. V. and Breitschwerdt, E. B. and Duncan, D. E.}, year={1991}, pages={429} }